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Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart

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Abstract

Apigenin (Api), a mainly bioactive component of Apium graveolens L. var. dulce DC. (a traditional Chinese medicinal herb), possesses a wide range of biological activities, including antioxidant effects. It also has been shown to associate with lower prevalence of cardiovascular diseases, but its mechanisms of action remain unclear. The aim of the present study is to investigate the role of Api in isolated rat heart model of ischemia/reperfusion (I/R). Langendorff-perfused isolated rat hearts were used in our study. Api was added to the perfusate before ischemia and during reperfusion in the isolated pulsed rat heart exposed to 30-min ischemia followed by 50-min reperfusion. The treatment with Api conferred a cardioprotective effect, and the treated hearts demonstrated an improved ischemic cardiac functional recovery, a decreased myocardial infarct size, a reduced activities of creatine kinase isoenzyme and lactate dehydrogenase in the coronary flow, a reduced number of apoptotic cardiomyocytes, a reduced activity of caspase-3, up-regulation of the anti-apoptotic protein Bcl-2 and down-regulation of the pro-apoptotic protein Bax. In addition, Api inhibited the phosphorylation of p38 MAPKS during I/R. In conclusion, these observations provide preliminary evidence that Api can protect cardiomyocytes from I-/R-induced injury, at least partially, through the inhibition of p38 MAPKS signaling pathway.

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Abbreviations

CK-MB:

Creatine kinase-MB isoform

I/R:

Ischemia–reperfusion

K–H:

Krebs–Henseleit

LDH:

Lactate dehydrogenase

TTC:

2,3,5-Triphenyltetrazolium chloride

LVEDP:

Left ventricular end-diastolic pressure

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Acknowledgments

We thank Xiao Cao Botanical Development Co., Ltd (Xi’an, China) for supplying us with the standardized Api extract used in this study. We are grateful for the financial support by National Natural Science Foundation of China (81400276).

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Pharmacy, General Hospital of Lanzhou Command, PLA, Lanzhou, 730050, China

    Jing Hu, Li-ting Xu, Ai-jun Sun, Li Zhang, Lin-lin Jing & Zheng-ping Jia

  2. Department of Cardiovascular Surgery, General Hospital of Lanzhou Command, PLA, Lanzhou, 730050, China

    Zilin Li, An-dong Lu & Yi-fei Dong

  3. Department of Ultrasound, General Hospital of Lanzhou Command, PLA, Lanzhou, 730050, China

    Xiao-yan Fu

Authors
  1. Jing Hu
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  2. Zilin Li
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  6. Li Zhang
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Corresponding authors

Correspondence to Yi-fei Dong or Zheng-ping Jia.

Additional information

Jing Hu, Zi-lin Li and Li-ting Xu have contributed equally to this work.

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Hu, J., Li, Z., Xu, Lt. et al. Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart. Cardiovasc Toxicol 15, 241–249 (2015). https://doi.org/10.1007/s12012-014-9290-y

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  • DOI: https://doi.org/10.1007/s12012-014-9290-y

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