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Glutathione Peroxidase 1, Selenoprotein K, and Selenoprotein H May Play Important Roles in Chicken Testes in Response to Selenium Deficiency

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Abstract

Selenium (Se) deficiency induces testicular functional disturbances, but the molecular mechanism remains unclear. In the present study, 1-day-old broiler chickens were maintained for 55 days with a normal diet (0.2 mg/kg) and a Se-deficient diet (0.033 mg Se/kg). Then, the messenger RNA (mRNA) levels of selenoproteins, heat shock proteins (HSPs), and inflammatory factors were examined. Se deficiency led to decreased selenoproteins (Gpx1, Selk, and Selh) and HSPs (HSP40, HSP60, and HSP90) (P < 0.05). However, the expression levels of Gpx2, Sepn1, Seli, Selpb, Sepx1, HSP27, and inflammatory factors (iNOS, TNF-α, COX-2, and HO-1) were increased by Se deficiency (P < 0.05). Gpx1, Selk, and Selh showed positive correlation with HSP40, HSP60, and HSP90, but negative correlation with HSP27, HSP70, iNOS, TNF-α, COX-2, and HO-1. However, Gpx2, Spen1, Seli, Selpb, and Sepx1 showed positive correlation with inflammatory factors and HSP27 and HSP70. Selenoproteins showed different correlation with HSPs and inflammatory factors and were classified into different groups in response to Se deficiency. The results suggested that selenoproteins play different roles in chicken testes, and we think that Gpx1 and Selk may play a special role in chicken testes.

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Acknowledgements

This study was supported by the Research Fund for the Doctoral Program of Higher Education (20122325110018), the National Natural Science Foundations of China (31472104, 31272626, and 30871902), and the National Science and Technology Support Program (2013BAD20B04). The authors thank the members in the Veterinary Internal Medicine Laboratory at the College of Veterinary Medicine, Northeast Agricultural University for their help in analyzing the data.

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Correspondence to Guixue Zhang.

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Gao, Y., Zhang, J., Huang, X. et al. Glutathione Peroxidase 1, Selenoprotein K, and Selenoprotein H May Play Important Roles in Chicken Testes in Response to Selenium Deficiency. Biol Trace Elem Res 179, 271–276 (2017). https://doi.org/10.1007/s12011-017-0953-y

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