Abstract
The purpose of this research was to evaluate the protective effects of apocynin on renal ischemia/reperfusion (I/R) injury (RI/RI) in rats. Rats preconditioned with apocynin were subjected to renal I/R. Zinc levels in serum and renal tissues, blood urea nitrogen (BUN), and serum creatinine (Scr) were detected. We further measured the activity of superoxide dismutase (SOD); the content of malondialdehyde (MDA), IL-4, IL-6, IL-10, and TNF-α; and the expression of metallothionein (MT) in the renal tissues. Results indicated that the levels of MDA, IL-4, IL-6, IL-10, TNF-α, and MT in the kidney tissue and serum BUN and Scr levels in RI/RI group were significantly higher than those in sham-operated group, while the levels of serum Zn and kidney Zn and SOD were reduced in RI/RI group. Apocynin treatment further decreased the levels of MDA, IL-6, TNF-α, and serum BUN and Scr, whereas it significantly increased the levels of Zn, SOD, IL-4, IL-10, and MT in the kidney tissue and serum Zn. These findings suggest that apocynin might play a protective role against RI/RI in rats through regulating zinc level and MT expression involving in oxidative stress.
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The authors appreciate the support from the financial support from the Science and Technology Planning Project of Zhejiang Province (2015C37130), the Zhejiang Provincial Natural Science Foundation (LQ14H230001), and the Science and Technology Planning Project of Jiaxing (2012AY1072-3).
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Bo Hu and Yuhong Wu contributed equally to this work.
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Hu, B., Wu, Y., Tong, F. et al. Apocynin Alleviates Renal Ischemia/Reperfusion Injury Through Regulating the Level of Zinc and Metallothionen. Biol Trace Elem Res 178, 71–78 (2017). https://doi.org/10.1007/s12011-016-0904-z
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DOI: https://doi.org/10.1007/s12011-016-0904-z