Skip to main content
SpringerLink
Log in

Cardioprotective Effect of Aloe vera Biomacromolecules Conjugated with Selenium Trace Element on Myocardial Ischemia-Reperfusion Injury in Rats

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

The present study was undertaken to evaluate the cardioprotection potential and underlying molecular mechanism afforded by a selenium (Se) polysaccharide (Se-AVP) from Aloe vera in the ischemia-reperfusion (I/R) model of rats in vivo. Myocardial I/R injury was induced by occluding the left anterior descending coronary artery (LAD) for 30 min followed by 2-h continuous reperfusion. Pretreatment with Se-AVP (100, 200, and 400 mg/kg) attenuated myocardial damage, as evidenced by reduction of the infarct sizes, increase in serum and myocardial endogenous antioxidants (superoxide dismutase (SOD), glutathione peroxidase (GSH), and catalase (CAT)), and decrease in the malondialdehyde (MDA) level in the rats suffering I/R injury. This cardioprotective activity afforded by Se-AVP is further supported by the decreased levels of cardiac marker enzymes creatine kinase (CK) and lactate dehydrogenase (LDH), as well as the rise of myocardial Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in I/R rats. Additionally, cardiomyocytic apoptosis was measured by terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) staining and the result showed that the percent of TUNEL-positive cells in myocardium of Se-AVP-treated groups was lower than I/R rats. In conclusion, we clearly demonstrated that Se-AVP had a protective effect against myocardial I/R injury in rats by augmenting endogenous antioxidants and protecting rat hearts from oxidative stress-induced myocardial apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Hansen PR (1995) Myocardial reperfusion injury: experimental evidence and clinical relevance. Eur Heart J 16:734–740

    Article  CAS  PubMed  Google Scholar 

  2. Visioli F, Keaney JF Jr, Halliwell B (2000) Antioxidant and cardiovascular disease; panacea or tonics for tired. Cardiovasc Res 47:409

    Article  CAS  PubMed  Google Scholar 

  3. Zweier JL (1988) Measurement of superoxide-derived free radicals in the reperfused heart. J Biol Chem 263:1353–1357

    CAS  PubMed  Google Scholar 

  4. Buttke TM, Sandstrom PA (1994) Oxidative stress as a mediator of apoptosis. Immunol Today 15:7–10

    Article  CAS  PubMed  Google Scholar 

  5. Chen EP, Bittner HB, Davis RD, Folz RJ, VanTrigt P (1996) Extracellular superoxide dismutase transgene overexpression preserves postischemic myocardial function in isolated murine hearts. Circulation 94:II412–II417

    CAS  PubMed  Google Scholar 

  6. Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL, Korsmeyer SJ (1993) Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell 75:241–251

    Article  CAS  PubMed  Google Scholar 

  7. Rodríguez ER, Darias JM, Díaz CR (2010) Aloe vera as a functional ingredient in foods. Crit Rev Food Sci Nutr 50:305–326

    Article  Google Scholar 

  8. Maze G, Terpolilli RN, Lee M (1997) Aloe vera extract prevents aspirin-induced acute gastric mucosal injury in rats. Med Sci Res 25:765–766

    CAS  Google Scholar 

  9. Heggers JP, Pelley RP, Robson MC (1993) Beneficial effects of Aloe in wound healing. Phytother Res 7:S48–S52

    Article  Google Scholar 

  10. Vázquez B, Avila G, Segura D, Escalante B (1996) Antiinflammatory activity of extracts from Aloe vera gel. J Ethnopharmacol 55:69–75

    Article  PubMed  Google Scholar 

  11. Mazzulla S, Sesti S, Schella A, Perrotta I, Anile A, Drogo S (2012) Protective effect of Aloe vera (Aloe barbadensis Miller) on erythrocytes anion transporter and oxidative change. Food Nutr Sci 3:1697–1702

    Article  CAS  Google Scholar 

  12. Volger BK, Ernest E (1999) Aloe vera: a systemic review of its clinical effectiveness. Br J Gen Pract 49:823–828

    Google Scholar 

  13. Hamman JH (2008) Composition and applications Aloe vera leaf gel. Molecules 13:1599–1616

    Article  CAS  PubMed  Google Scholar 

  14. Kaithwas G, Singh P, Bhatia D (2014) Evaluation of in vitro and in vivo antioxidant potential of polysaccharides from Aloe vera (Aloe barbadensis Miller) gel. Drug Chem Toxicol 37:135–143

    Article  CAS  PubMed  Google Scholar 

  15. Liu CH, Wang CH, Xu ZL, Wang Y (2007) Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water. Process Biochem 42:961–970

    Article  CAS  Google Scholar 

  16. Staub AM (1965) Removal of protein-Sevag method. Methods Carbohydr Chem 5:5–6

    CAS  Google Scholar 

  17. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  18. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  19. Filisetti-Cozzi TMCC, Carpita NC (1991) Measurement of uronic acids without interference from neutral sugars. Anal Biochem 197:157–162

    Article  CAS  PubMed  Google Scholar 

  20. Gao Z, Chen J, Qiu S, Li Y, Wang D, Liu C, Li X, Hou R, Yue C, Liu J, Li H, Hu Y (2016) Optimization of selenylation modification for garlic polysaccharide based on immune-enhancing activity. Carbohydr Polym 136:560–569

    Article  CAS  PubMed  Google Scholar 

  21. Sun Y, Wang S, Li T, Li X, Jiao L, Zhang L (2008) Purification, structure and immunobiological activity of a new water-soluble polysaccharide from the mycelium of Polyporus albicans (Imaz.) Teng. Bioresour Technol 99:900–904

    Article  CAS  PubMed  Google Scholar 

  22. Jones TM, Albersheim P (1972) A gas chromatographic method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharides. Plant Physiol 49:926–936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Oades JM (1967) Gas–liquid chromatography of alditol acetates and its application to the analysis of sugars in complex hydrolysates. J Chromatogr 28:246–252

    Article  CAS  PubMed  Google Scholar 

  24. Song M, Huang L, Zhao G, Song Y (2013) Beneficial effects of a polysaccharide from Salvia miltiorrhiza on myocardial ischemia-reperfusion injury in rats. Carbohydr Polym 98:1631–1636

    Article  CAS  PubMed  Google Scholar 

  25. Kloner RA, Rude RE, Carlson N, Maroko PR, DeBoer LW, Braunwald E (1980) Ultrastructural evidence of microvascular damage and myocardial cell injury after coronary artery occlusion: which comes first. Circulation 62:945–952

    Article  CAS  PubMed  Google Scholar 

  26. Adegunloye BJ, Omoniyi JO, Owolabi OA, Ajagbonna OP, Sofola OA, Coker HAB (1996) Mechanisms of the blood pressure lowering effect of the calyx extract of Hibiscus sabdariffa in rats. Afr J Med Sci 25:235–238

    CAS  Google Scholar 

  27. Onyenekwe PC, Ajani EO, Amechi DA, Garnamel KS (1999) Antihypertensive effect of Rosselle (Hibiscus sabdariffa) calyx infusion in spontaneously hypertensive rats and a comparison of its toxicity with that in Wistar rats. Cell Biochem Funct 17:199–205

    Article  CAS  PubMed  Google Scholar 

  28. Zhao W, Li JJ, Yue SQ, Zhang LY, Dou KF (2012) Antioxidant activity and hepatoprotective effect of a polysaccharide from Bei Chaihu (Bupleurum chinense DC). Carbohydr Polym 89:448–452

    Article  CAS  PubMed  Google Scholar 

  29. Yan B, Jing L, Wang J (2015) A polysaccharide (PNPA) from Pleurotus nebrodensis offers cardiac protection against ischemia–reperfusion injury in rats. Carbohydr Polym 133:1–7

    Article  CAS  PubMed  Google Scholar 

  30. Parlakpinar H, Ozer MK, Acet A (2005) Effect of aminoguanidine on ischemia-reperfusion induced myocardial injury in rats. Mol Cell Biochem 277:137–142

    Article  CAS  PubMed  Google Scholar 

  31. Basaga HS (1990) Biochemical aspects of free radicals. Biochem Cell Biol 68:989–998

    Article  CAS  PubMed  Google Scholar 

  32. Bansal P, Gupta SK, Ojha SK, Nandave M, Mittal R, Kumari S, Arya DS (2006) Cardioprotective effect of lycopene in the experimental model of myocardial ischemia-reperfusion injury. Mol Cell Biochem 289:1–9

    Article  CAS  PubMed  Google Scholar 

  33. Zhang J, Wang H, Zheng Q (2013) Cardioprotective effect of Aralia elata polysaccharide on myocardial ischemic reperfusion (IR) injury in rats. Int J Biol Macromol 59:328–332

    Article  CAS  PubMed  Google Scholar 

  34. Ke YS, Wang DG, Wang HG, Yang SY (2004) Endoxin antagonist lessens myocardial ischemia reperfusion injury. Cardiovasc Drugs Ther 18:289–293

    Article  CAS  PubMed  Google Scholar 

  35. Bassenge E1, Sommer O, Schwemmer M, Bünger R (2000) Antioxidant pyruvate inhibits cardiac formation of reactive oxygen species through changes in redox state. [J] Am J Physiol Heart Circ Physiol 279:H2431–H2438

    CAS  PubMed  Google Scholar 

  36. Abbate A, Melfi R, Patti G, Baldi F, D’Ambrosio A, Manzoli A, Baldi A, Di Sciascio G (2000) Apoptosis in recent myocardial infarction. Clin Ther 151:247–251

    CAS  Google Scholar 

  37. Narula J, Pandey P, Arbustini E, Haider N, Narula N, Kolodgie FD, Dal Bello B, Semigran MJ, Bielsa-Masdeu A, Dec GW, Israels S, Ballester M, Virmani R, Saxena S, Kharbanda S (1999) Apoptosis in heart failure: release of cytochrome c from mitochondria and activation of caspase-3 in human cardiomyopathy. Proc Natl Acad Sci 96:8144–8149

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Kajstura J, Cheng W, Reiss K, Clark WA, Sonnenblick EH, Krajewski S, Reed JC, Olivetti G, Anversa P (1996) Apoptotic and necrotic myocyte cell deaths are independent contributing variables of infarct size in rats. Lab Investig 74:86–107

    CAS  PubMed  Google Scholar 

  39. Toufektsian MC, Boucher F, Pucheu S, Tanguy S, Ribuot C, Sanou D, Tresallet N, de Leiris J (2000) Effects of selenium deficiency on the response of cardiac tissue to ischemia and reperfusion. Toxicology 148:125–132

    Article  CAS  PubMed  Google Scholar 

  40. Turan B, Saini HK, Zhang M, Prajapati D, Elimban V, Dhalla NS (2005) Selenium improves cardiac function by attenuating the activation of NF-κB due to ischemia-reperfusion injury. Antioxid Redox Signal 7:1388–1397

    Article  CAS  PubMed  Google Scholar 

  41. Soumya RS, Vineetha VP, Salin Raj P, Raghu KG (2014) Beneficial properties of selenium incorporated guar gum nanoparticles against ischemia/reperfusion in cardiomyoblasts (H9c2). Metallomics 6:2134–2147

  42. Venardos KM, Perkins A, Headrick J, Kaye DM (2007) Myocardial ischemia-reperfusion injury, antioxidant enzyme systems, and selenium: a review. Curr Med Chem 14:1539–1549

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, China

    Yang Yang & Congxin Huang

  2. Department of Network Medical, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China

    Yang Yang & Ming Yang

  3. Emergency Department, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China

    Fen Ai

Authors
  1. Yang Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ming Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fen Ai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Congxin Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Congxin Huang.

Ethics declarations

All experimental procedures were approved by the institutional Animal Ethics Committee of Wuhan University.

Conflict of Interest

The authors declare no conflict of interest. All authors have read and approved the final manuscript.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yang, Y., Yang, M., Ai, F. et al. Cardioprotective Effect of Aloe vera Biomacromolecules Conjugated with Selenium Trace Element on Myocardial Ischemia-Reperfusion Injury in Rats. Biol Trace Elem Res 177, 345–352 (2017). https://doi.org/10.1007/s12011-016-0896-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-016-0896-8

Keywords

Search

Navigation