Abstract
Camel tear metabolomics and elemental analysis are useful in getting the information regarding the components responsible for maintaining the protective system that allows living in the desert and dry regions. The aim of this study was to correlate that the camel tears can be used as artificial tears for the evaluation of dryness in the eye. Eye biomarkers of camel tears were analyzed by gas chromatography-mass spectroscopy (GC-MS) and inductively coupled plasma mass spectroscopy (ICP-MS). The major compounds detected in camel tears by GC-MS were alanine, valine, leucine, norvaline, glycine, cadaverine, urea, ribitol, sugars, and higher fatty acids like octadecanoic acid and hexadecanoic acid. GC-MS analysis of camel tears also finds several products of metabolites and its associated metabolic participants. ICP-MS analysis showed the presence of different concentration of elemental composition in the camel tears.
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Al-Shehri AM (2015) A lesson learned from Middle East respiratory syndrome (MERS) in Saudi Arabia. Med Teach 37:S88–S93
Al-Ruwaili MA, Khalil OM, Selim SA (2012) Viral and bacterial infections associated with camel (Camelus dromedarius) calf diarrhea in North Province, Saudi Arabia. Saudi J Biol Sci 19:35–41
Holly FJ, Lemp MA (1977) Tear physiology and dry eyes. Surv Ophthalmol 22:69–87
Redl B (2000) Human tear lipocalin. Biochim Biophys Acta 1482:241–248
Lehrer RI, Xu G, Abduragimov A, Dinh NN, Qu XD, Martin D, Glasgow BJ (1998) Lipophilin, a novel heterodimeric protein of human tears. FEBS Lett 432:163–167
Grus FH, Podust VN, Bruns K, Lackner K, Fu S, Dalmasso EA, Wirthlin A, Pfeiffer N (2005) SELDI-TOFMS protein chip array profiling of tears from patients with dry eye. Invest Ophthalmol Vis Sci 46:863–876
Zhou L, Huang LQ, Beuerman RW, Grigg ME, Li SF, Chew FT, Ang L, Stem ME, Tan D (2004) Proteomic analysis of human tears: defensin expression after ocular surface surgery. J Proteome Res 3:410–416
Zhou L, Beuerman RW, Barathi A, Tan D (2003) Analysis of rabbit tear proteins by high-pressure liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 17:401–412
Fung KY, Morris C, Sathe S, Sack R, Duncan MW (2004) Characterization of the in vivo forms of lacrimal-specific proline-rich proteins in human tear fluid. Proteomics 4:3953–3959
de Souza GA, Godoy LM, Mann M (2006) Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors. Genome Biol 7:R72
Molloy MP, Bolis S, Herbert BR, Ou K, Tyler MI, van Dyk DD, Willcox MD, Gooley AA, Williamd KL, Morris CA, Walsh BJ (1997) Establishment of the human reflex tear two-dimensional polyacrylamide gel electrophoresis reference map: new proteins potential diagnostic value. Electrophoresis 18:2811–2815
Grus FH, Augustin AJ (2001) High performance liquid chromatography analysis of tear protein patterns in diabetic and non-diabetic dry-eye patients. Eur J Ophthalmol 11:19–24
McCulley JP, Shine WE (2003) Meibomian gland function and the tear lipid layer. Ocul Surf 1:97–106
Butovich IA, Millar TJ, Ham BM (2008) Understanding and analyzing Meibomian lipids—a review. Curr Eye Res 33:405–420
Lindon JC, Nicholson JK (2008) Analytical technologies for metabonomics and metabolomics, and multi-omic information recovery. Trend Anal Chem 27:194–204
Lenz EM (2011) Nuclear magnetic resonance (NMR)-based drug metabolite profiling. Methods Mol Biol 708:299–319
Ası T. Tablolarla Biyokimya. Ankara, Turkey: Nobel Tıp Kitapları Dağıtım (in Turkish)
Haenlein GFW, Anke M (2011) Mineral and trace element research in goats: a review. Small Ruminant Res 95:2–19
Hefnawy AEG, Tortora-Pérez JL (2010) The importance of selenium and the effects of its deficiency in animal health. Small Ruminant Res 89:185–192
Ahamad SR, Raish M, Ahmad A, Shakeel F (2016) Potential health benefits and metabolomics of camel milk by GC-MS and ICP-MS. Biol Trace Elem Res. doi:10.1007/s12011-016-0771-7
Raish M, Ahmad A, Alkharfy KM, Al-Jenoobi FI, Al-Mohizea AM, Mohsin K, Ahamad SR, Ali M, Shakeel F (2016) Antioxidant potential and in situ analysis of major and trace element determination of Ood-saleeb, a known Unani herbal medicine by ICP-MS. Biol Trace Elem Res 172:521–527
Koenig R (2007) Veterinary medicine. “Camelized” antibodies make waves. Science 318:1373
Shamsi FA, Chen Z, Liang J, Li K, Al-Rajhi AA, Chaudhry IA, Li M, Wu K (2011) Analysis and comparison of proteomic profiles of tear fluid from human, cow, sheep, and camel eyes. Inves Ophthalmol Vis Sci 52:9156–9165
Bacha AB, Abid I (2013) Secretory phospholipase A in dromedary tears: a host defense against staphylococci and other gram-positive bacteria. Appl Biochem Biotechnol 169:1858–1869
The epidemiology of dry eye disease: report of the Epidemiology Subcommittee of the International Dry Eye Workshop (2007). Ocul Surf 5: 93–107
Lu P, Chen X, Liu X, Yu L, Kang Y, Xie Q, Ke L, Wei X (2008) Dry eye syndrome in elderly Tibetans at high altitude: a population-based study in China. Cornea 27:545–551
Wolkoff P (2010) Ocular discomfort by environmental and personal risk factors altering the precorneal tear film. Toxicol Lett 199:203–212
Motamedifar M, Bazargani A, Reza M, Sarai E, Sedigh H (2014) Antimicrobial activity of mandelic acid against methicillin-resistant Staphylococcus aureus: a novel finding with important practical implications. World Appl Sci J 31:925–929
Inaba T, Hisatsune C, Sasaki Y, Ogawa Y, Ebisui E, Ogawa N, Matsui M, Takeuchi T, Mikoshiba K, Tsubota K (2014) Mice lacking inositol 1,4,5-trisphosphate receptors exhibit dry eye. PLoS One 9:e99205
Tsukamoto S, Fujiwara K, Ikeguchi M (2009) Fatty acids bound to recombinant tear lipocalin and their role in structural stabilization. J Biochem 146:343–350
Simonsen LO, Harbak H, Bennekou P (2012) Cobalt metabolism and toxicology—a brief update. Sci Total Environ 432:210–215
Ayiannidis A, Giroussi S, Voulgaropoulos A (1996) Aspects of protein bound copper in sheep plasma and its release in vitro especially after treatment with ammonium tetrathiomolybdate. J Trace Elements Med Biol 10:245–250
Patching SG, Gardiner PH (1999) Recent developments in selenium metabolism and chemical speciation: a review. J Trace Elements Med Biol 13:193–214
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The authors extend their sincere appreciation to the Research Center, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia, for funding this project.
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Ahamad, S.R., Raish, M., Yaqoob, S.H. et al. Metabolomics and Trace Element Analysis of Camel Tear by GC-MS and ICP-MS. Biol Trace Elem Res 177, 251–257 (2017). https://doi.org/10.1007/s12011-016-0889-7
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DOI: https://doi.org/10.1007/s12011-016-0889-7