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The Changes of Zinc Transporter ZnT Gene Expression in Response to Zinc Supplementation in Obese Women

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Abstract

Obesity is associated with an alteration in zinc metabolism. This alteration may be associated with changes in gene expression of zinc transporters. In this study, we examined the leukocyte expression of zinc transporter ZnTs in response to zinc supplementation in young obese women. Thirty-five young obese women (BMI ≥ 25 kg/m2), aged 18–28 years, were randomly assigned to two groups: a placebo group or a zinc group (30 mg zinc/day for 8 weeks). Usual dietary zinc intake was estimated from 3-day diet records. Serum zinc and urinary zinc concentrations were measured by atomic absorption spectrometry. Messenger RNA (mRNA) levels of leukocyte ZnT transporters were examined using quantitative real-time PCR. Expression levels of two ZnT transporters, ZnT1 and ZnT5, in obese women, increased significantly after zinc supplementation. At the end of the study, mRNA levels of ZnT1 and ZnT5 showed no correlation with serum zinc or urinary zinc concentration in obese women. In addition, a further study was conducted to identify whether the association between the gene expression levels of leukocyte ZnT1 and ZnT5 and dietary zinc intake remained consistent in 216 healthy young adults aged 20–29 years. A positive correlation between ZnT1 and dietary zinc intake (r = 0.181, P = 0.089) was also observed in healthy men although the significance was marginal. Taken together, these results show that the gene expression levels of ZnT1 and ZnT5 may be changed by zinc intake, suggesting that zinc supplementation could potentially restore ZnT transporter expression in obese women with altered zinc metabolism.

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References

  1. Brown KH, Rivera JA, Bhutta Z, Gibson RS, King JC, Lonnerdal B, Ruel MT, Sandtrom B, Wasantwisut E, Hotz C (2004) International Zinc Nutrition Consultative Group (IZiNCG) technical document #1. Assessment of the risk of zinc deficiency in populations and options for its control. Food Nutr Bull 25(1 Suppl 2):S99–S203

    PubMed  Google Scholar 

  2. Liuzzi JP, Cousins RJ (2004) Mammalian zinc transporters. Annu Rev Nutr 24:151–172

    Article  CAS  PubMed  Google Scholar 

  3. Lichten LA, Cousins RJ (2009) Mammalian zinc transporters: nutritional and physiologic regulation. Annu Rev Nutr 29:153–176

    Article  PubMed  Google Scholar 

  4. Huang L, Tepaamorndech S (2013) The SLC30 family of zinc transporters—a review of current understanding of their biological and pathophysiological roles. Mol Aspects Med 34(2–3):548–560

    Article  CAS  PubMed  Google Scholar 

  5. Noh H, Paik HY, Kim J, Chung J (2014) The alteration of zinc transporter gene expression is associated with inflammatory markers in obese women. Biol Trace Elem Res 158(1):1–8

    Article  CAS  PubMed  Google Scholar 

  6. McMahon RJ, Cousins RJ (1998) Regulation of the zinc transporter ZnT-1 by dietary zinc. Proc Natl Acad Sci U S A 95(9):4841–4846

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Liuzzi JP, Blanchard RK, Cousins RJ (2001) Differential regulation of zinc transporter 1, 2, and 4 mRNA expression by dietary zinc in rats. J Nutr 131(1):46–52

    CAS  PubMed  Google Scholar 

  8. Cousins RJ, Blanchard RK, Popp MP, Liu L, Cao J, Moore JB, Green CL (2003) A global view of the selectivity of zinc deprivation and excess on genes expressed in human THP-1 mononuclear cells. Proc Natl Acad Sci U S A 100(12):6952–6957

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Andree KB, Kim J, Kirschke CP, Gregg JP, Paik H, Joung H, Woodhouse L, King JC, Huang L (2004) Investigation of lymphocyte gene expression for use as biomarkers for zinc status in humans. J Nutr 134(7):1716–1723

    CAS  PubMed  Google Scholar 

  10. Overbeck S, Uciechowski P, Ackland ML, Ford D, Rink L (2008) Intracellular zinc homeostasis in leukocyte subsets is regulated by different expression of zinc exporters ZnT-1 to ZnT-9. J Leukoc Biol 83(2):368–380

    Article  CAS  PubMed  Google Scholar 

  11. Aydemir TB, Blanchard RK, Cousins RJ (2006) Zinc supplementation of young men alters metallothionein, zinc transporter, and cytokine gene expression in leukocyte populations. Proc Natl Acad Sci U S A 103(6):1699–1704

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Ezzati M, Lopez AD, Rodgers A, Vander Hoorn S, Murray CJ, Comparative Risk Assessment Collaborating G (2002) Selected major risk factors and global and regional burden of disease. Lancet 360(9343):1347–1360

    Article  PubMed  Google Scholar 

  13. Caballero B (2007) The global epidemic of obesity: an overview. Epidemiol Rev 29:1–5

    Article  PubMed  Google Scholar 

  14. Astrup A, Bugel S (2010) Micronutrient deficiency in the aetiology of obesity. Int J Obes 34(6):947–948

    Article  CAS  Google Scholar 

  15. Tungtrongchitr R, Pongpaew P, Phonrat B, Tungtrongchitr A, Viroonudomphol D, Vudhivai N, Schelp FP (2003) Serum copper, zinc, ceruloplasmin and superoxide dismutase in Thai overweight and obese. J Med Assoc Thai 86(6):543–551

    PubMed  Google Scholar 

  16. Marreiro DN, Fisberg M, Cozzolino SM (2004) Zinc nutritional status and its relationships with hyperinsulinemia in obese children and adolescents. Biol Trace Elem Res 100(2):137–149

    Article  PubMed  Google Scholar 

  17. Ennes Dourado Ferro F, de Sousa Lima VB, Mello Soares NR, Franciscato Cozzolino SM, do Nascimento Marreiro D (2011) Biomarkers of metabolic syndrome and its relationship with the zinc nutritional status in obese women. Nutr Hosp 26(3):650–654

    CAS  PubMed  Google Scholar 

  18. Kim J, Ahn J (2014) Effect of zinc supplementation on inflammatory markers and adipokines in young obese women. Biol Trace Elem Res 157(2):101–106

    Article  PubMed  Google Scholar 

  19. Smidt K, Pedersen SB, Brock B, Schmitz O, Fisker S, Bendix J, Wogensen L, Rungby J (2007) Zinc-transporter genes in human visceral and subcutaneous adipocytes: lean versus obese. Mol Cell Endocrinol 264(1–2):68–73

    Article  CAS  PubMed  Google Scholar 

  20. Burczynski ME, Dorner AJ (2006) Transcriptional profiling of peripheral blood cells in clinical pharmacogenomic studies. Pharmacogenomics 7(2):187–202

    Article  CAS  PubMed  Google Scholar 

  21. Sagaya FM, Hurrell RF, Vergeres G (2012) Postprandial blood cell transcriptomics in response to the ingestion of dairy products by healthy individuals. J Nutr Biochem 23(12):1701–1715

    Article  CAS  PubMed  Google Scholar 

  22. Lonnerdal B (2000) Dietary factors influencing zinc absorption. J Nutr 130(5S Suppl):1378S–1383S

    CAS  PubMed  Google Scholar 

  23. Kim J, Paik HY, Joung H, Woodhouse LR, Li S, King JC (2004) Zinc supplementation reduces fractional zinc absorption in young and elderly Korean women. J Am Coll Nutr 23(4):309–315

    Article  CAS  PubMed  Google Scholar 

  24. Jacobs RM, Lumsden JH, Taylor JA, Grift E (1991) Effects of interferents on the kinetic Jaffe reaction and an enzymatic colorimetric test for serum creatinine concentration determination in cats, cows, dogs and horses. Can J Vet Res 55(2):150–154

    CAS  PubMed Central  PubMed  Google Scholar 

  25. Noh H, Paik HY, Kim J, Chung J (2013) Salty taste acuity is affected by the joint action of alphaENaC A663T gene polymorphism and available zinc intake in young women. Nutrients 5(12):4950–4963

    Article  PubMed Central  PubMed  Google Scholar 

  26. Foster M, Petocz P, Samman S (2013) Inflammation markers predict zinc transporter gene expression in women with type 2 diabetes mellitus. J Nutr Biochem 24(9):1655–1661

    Article  CAS  PubMed  Google Scholar 

  27. Kim J, Paik HY, Joung H, Woodhouse LR, Li S, King JC (2007) Effect of dietary phytate on zinc homeostasis in young and elderly Korean women. J Am Coll Nutr 26(1):1–9

    Article  CAS  PubMed  Google Scholar 

  28. Kambe T, Narita H, Yamaguchi-Iwai Y, Hirose J, Amano T, Sugiura N, Sasaki R, Mori K, Iwanaga T, Nagao M (2002) Cloning and characterization of a novel mammalian zinc transporter, zinc transporter 5, abundantly expressed in pancreatic beta cells. J Biol Chem 277(21):19049–19055

    Article  CAS  PubMed  Google Scholar 

  29. Suzuki T, Ishihara K, Migaki H, Ishihara K, Nagao M, Yamaguchi-Iwai Y, Kambe T (2005) Two different zinc transport complexes of cation diffusion facilitator proteins localized in the secretory pathway operate to activate alkaline phosphatases in vertebrate cells. J Biol Chem 280(35):30956–30962

    Article  CAS  PubMed  Google Scholar 

  30. Jackson KA, Valentine RA, Coneyworth LJ, Mathers JC, Ford D (2008) Mechanisms of mammalian zinc-regulated gene expression. Biochem Soc Trans 36(Pt 6):1262–1266

    Article  CAS  PubMed  Google Scholar 

  31. Cragg RA, Christie GR, Phillips SR, Russi RM, Kury S, Mathers JC, Taylor PM, Ford D (2002) A novel zinc-regulated human zinc transporter, hZTL1, is localized to the enterocyte apical membrane. J Biol Chem 277(25):22789–22797

    Article  CAS  PubMed  Google Scholar 

  32. Langmade SJ, Ravindra R, Daniels PJ, Andrews GK (2000) The transcription factor MTF-1 mediates metal regulation of the mouse ZnT1 gene. J Biol Chem 275(44):34803–34809

    Article  CAS  PubMed  Google Scholar 

  33. Kim J, Paik HY, Joung H, Woodhouse LR, King JC (2011) Plasma zinc but not the exchangeable zinc pool size differs between young and older Korean women. Biol Trace Elem Res 142(2):130–136

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) founded by the Ministry of Education, Science and Technology (NRF-2010-0011226 and NRF2012R1A1A1012317).

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Correspondence to Jihye Kim or Jayong Chung.

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Noh, H., Paik, H.Y., Kim, J. et al. The Changes of Zinc Transporter ZnT Gene Expression in Response to Zinc Supplementation in Obese Women. Biol Trace Elem Res 162, 38–45 (2014). https://doi.org/10.1007/s12011-014-0128-z

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  • DOI: https://doi.org/10.1007/s12011-014-0128-z

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