Abstract
The myeloperoxidase (MPO) activity and its corresponding mRNA expression as well as gene polymorphism were investigated in the population who live in the endemic fluorosis area. In the study, 150 people were selected from the coal-burning endemic fluorosis area and 150 normal persons from the non-fluorosis area in Guizhou province of China. The blood samples were collected from these people. The activity of MPO in the plasma was determined by spectrophotometer; the expression of MPO mRNA was measured by employing real-time polymerase chain reaction; DNAs were extracted from the leucocytes in blood and five SNP genotypes of MPO promoter gene detected by a multiplex genotyping method, adapter-ligation-mediated allele-specific amplification. The results showed that the MPO activity and its corresponding mRNA in blood were significantly increased in the population living in the area of fluorosis. The different genotype frequencies of MPO, including –1228G/A, –585T/C, –463G/A, and –163C/T, and the three haplotypes with higher frequencies, including –163C–463G–585T–1228G–1276T, –163C–463G–585T–1228G–1276C, and –163C–463G–585T–1228A–1276T, were significantly associated with fluorosis. The results indicated that the elevated activity of MPO induced by endemic fluorosis may be connected in mechanism to the stimulated expression of MPO mRNA and the changed gene polymorphism.
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Acknowledgments
This work was financed by grants from the China National Natural Scientific Foundation (81160335), the Foundation in the Ministry of Science and Technology of China (2010DFB30530 and 2011BAZ03220), and the Government Foundations in Guizhou Province of China [G(2011)7014 and S201104].
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Zhang, T., Shan, KR., Tu, X. et al. Myeloperoxidase Activity and Its Corresponding mRNA Expression as well as Gene Polymorphism in the Population Living in the Coal-Burning Endemic Fluorosis Area in Guizhou of China. Biol Trace Elem Res 152, 379–386 (2013). https://doi.org/10.1007/s12011-013-9632-9
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DOI: https://doi.org/10.1007/s12011-013-9632-9