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Myeloperoxidase Activity and Its Corresponding mRNA Expression as well as Gene Polymorphism in the Population Living in the Coal-Burning Endemic Fluorosis Area in Guizhou of China

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Abstract

The myeloperoxidase (MPO) activity and its corresponding mRNA expression as well as gene polymorphism were investigated in the population who live in the endemic fluorosis area. In the study, 150 people were selected from the coal-burning endemic fluorosis area and 150 normal persons from the non-fluorosis area in Guizhou province of China. The blood samples were collected from these people. The activity of MPO in the plasma was determined by spectrophotometer; the expression of MPO mRNA was measured by employing real-time polymerase chain reaction; DNAs were extracted from the leucocytes in blood and five SNP genotypes of MPO promoter gene detected by a multiplex genotyping method, adapter-ligation-mediated allele-specific amplification. The results showed that the MPO activity and its corresponding mRNA in blood were significantly increased in the population living in the area of fluorosis. The different genotype frequencies of MPO, including –1228G/A, –585T/C, –463G/A, and –163C/T, and the three haplotypes with higher frequencies, including –163C–463G–585T–1228G–1276T, –163C–463G–585T–1228G–1276C, and –163C–463G–585T–1228A–1276T, were significantly associated with fluorosis. The results indicated that the elevated activity of MPO induced by endemic fluorosis may be connected in mechanism to the stimulated expression of MPO mRNA and the changed gene polymorphism.

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Reference

  1. Li JX, Cao SR (1994) Recent studies on endemic fluorosis in China. Fluoride 27:125–128

    Google Scholar 

  2. Zhou YX, Mo SL, Wang RH et al (2012) Corn baked by burning coal triggered overexpression of osteopontin in hepatocytes of rats following fluorosis. Toxicol Ind Health 28:195–202

    Article  PubMed  CAS  Google Scholar 

  3. Yan XY, Yan XT, Morrison A et al (2011) Fluoride induces apoptosis and alters collagen I expression in rat osteoblasts. Toxicol Lett 200:133–138

    Article  PubMed  CAS  Google Scholar 

  4. Zahvoronkov AA, Strochkova LS (1981) Fluorosis: geographical pathology and some experimental findings. Fluoride 14:182–191

    Google Scholar 

  5. Sun DJ, Gao YH, Yu GQ (2006) The discuss of prophylaxis and research in endemic fluorosis and arseniasis during National Eleventh Five-Year Plan of China. Chin J Endemiol 25:3–5

    CAS  Google Scholar 

  6. Gao Q, Liu YJ, Guan ZZ (2008) Oxidative stress might be a mechanism connected with the decreased α7 nicotinic receptor influenced by high-concentration of fluoride in SH-SY5Y neuroblastoma cells. Toxicol in Vitro 22:837–843

    Article  PubMed  CAS  Google Scholar 

  7. Guan ZZ, Xiao KQ, Zeng XY et al (2000) Changed cellular membrane lipid composition and lipid peroxidation of kidney in rats with chronic fluorosis. Arch Toxicol 74:602–608

    Article  PubMed  CAS  Google Scholar 

  8. Gao Q, Liu YJ, Guan ZZ (2009) Decreased learning and memory ability in rats with fluorosis: increased oxidative stress and reduced cholinesterase activity in the brain. Fluoride 42:277–285

    CAS  Google Scholar 

  9. Liu GY, Chai CY, Cui L (2003) Fluoride causing abnormally elevated serum nitric oxide levels in chicks. Environ Toxicol Pharmacol 13:199–204

    Article  PubMed  CAS  Google Scholar 

  10. Maruyama Y, Lindholm B, Stenvinkel P (2004) Inflammation and oxidative stress in ESRD—the role of myeloperoxidase. J Nephrol 17:S72–76

    PubMed  CAS  Google Scholar 

  11. Wu AHB (2009) Novel biomarkers of cardiovascular disease: myeloperoxidase for acute and/or chronic heart failure? Clin Chem 55:12–14

    Article  PubMed  CAS  Google Scholar 

  12. Klebanoff SJ, Waltersdorph AM, Rosen H (1984) Antimicrobial activity of myeloperoxidase. Method Enzymol 105:399–403

    Article  CAS  Google Scholar 

  13. Zhang R, Brennan ML, Shen Z et al (2002) Myeloperoxidase functions as a major enzymatic catalyst for initiation of lipid peroxidation at sites of inflammation. J Biol Chem 277:46116–46122

    Article  PubMed  CAS  Google Scholar 

  14. Reynolds WF, Chang E, Douer D et al (1997) An allelic association implicates myeloperoxidase in the etiology of acute promyelocytic leukemia. Blood 90:2730–2737

    PubMed  CAS  Google Scholar 

  15. Nikpoor B, Turecki G, Fournier C et al (2001) A functional myeloperoxidase polymorphic variant is associated with coronary artery disease in French-Canadians. Am Heart J 142:336–339

    Article  PubMed  CAS  Google Scholar 

  16. Piedrafita FJ, Molander RB, Vansant G et al (1996) An Alu element in the myeloperoxidase promoter contains a composite SP1-thyroid hormone-retinoic acid response element. J Biol Chem 271:14412–14420

    Article  PubMed  CAS  Google Scholar 

  17. Wang WP, Ni KY, Zhou GH (2007) Association of IL1B polymorphisms with gastric cancer in a Chinese population. Clin Biochem 40:218–225

    Article  PubMed  CAS  Google Scholar 

  18. Toper R, Aviram A, Aviram I (1987) Fluoride mediated activation of guinea pig neutrophils. Biochem Biophys Acta—Mol Cell Res 931:262–266

    Article  CAS  Google Scholar 

  19. Luo KL, Li L, Zhang SX (2011) Coal-burning roasted corn and chili as the cause of dental fluorosis for children in southwestern China. J Hazard Mater 185:1340–1347

    Article  PubMed  CAS  Google Scholar 

  20. Zhong H, Yu F, Yi X (1979) Endemic food-born fluorosis in Guizhou. Chin J Prev Med (Chinese) 13:148–151

    Google Scholar 

  21. Wang YN, Xiao KQ, Liu JL et al (2000) Effect of long term fluoride exposure on lipid composition in rat liver. Toxicology 146:161–169

    Article  PubMed  CAS  Google Scholar 

  22. Feng P, Wei J, Zhang Z (2011) Intervention of selenium on chronic fluorosis-induced injury of blood antioxidant capacity in rats. Biol Trace Elem Res 144(1–3):1024–1031

    Article  PubMed  CAS  Google Scholar 

  23. Guan ZZ, Yang PS, Pan S et al (1989) An experimental study of blood biochemical diagnostic indices for chronic fluorosis. Fluoride 22:112–118

    CAS  Google Scholar 

  24. Borawski J (2006) Myeloperoxidase as a morker of hemodialysis biocompatibility and oxidative stress: the underestimated modifying effects of heparin. Am J Kidney Dis 47:37–41

    Article  PubMed  Google Scholar 

  25. Pecoits-Filho R, Stenvinkel P, Marchlewska A et al (2003) A functional variant of the myeloperoxidase gene is associated with cardiovascular disease in end-stage renal disease patients. Kidney Int 63:S172–176

    Article  Google Scholar 

  26. La Rocca G, Di Stefano A, Eleuteri E et al (2009) Oxidative stress induces myeloperoxidase expression in endocardial endothelial cells from patients with chronic heart failure. Basic Res Cardiol 104:307–320

    Article  PubMed  CAS  Google Scholar 

  27. Nakazato T, Sagawa M, Yamato K et al (2007) Myeloperoxidase is a key regulator of oxidative stress mediated apoptosis in myeloid leukemic cells. Clin Cancer Res 13:5436–5445

    Article  PubMed  CAS  Google Scholar 

  28. Mäkelä R, Karhunen PJ, Kunnas TA et al (2003) Myeloperoxidase gene variation as a determinant of atherosclerosis progression in the abdominal and thoracic aorta: an autopsy study. Lab Invest 83:919–925

    Article  PubMed  Google Scholar 

  29. Mäkelä R, Dastidar P, Jokela H et al (2003) Effect of long-term hormone replacement therapy on atherosclerosis progression in postmenopausal women relates to myeloperoxidase promoter polymorphism. J Clin Endocrinol Metab 88(8):3823–3828

    Article  PubMed  Google Scholar 

  30. Chumakov AM, Chumakova EA, Chih D et al (2000) Molecular analysis of the human myeloperoxidase promoter region. Int J Oncol 16:401–411

    PubMed  CAS  Google Scholar 

  31. Reynolds WF, Chang E, Douer D et al (1997) An allelic association implicates myeloperoxidase in the etiology of acute promyelocytic leukemia. Blood 90:2730–2737

    PubMed  CAS  Google Scholar 

  32. Vaccarino V, Brennan ML, Miller AH et al (2008) Association of major depressive disorder with serum myeloperoxidase and other markers of inflammation: a twin study. Biol Psychiat 64:476–483

    Article  PubMed  CAS  Google Scholar 

  33. Reynolds WF, Rhees J, Maciejewski D et al (1999) Myeloperoxidase polymorphism is associated with gender specific risk for Alzheimer’s disease. Exp Neurol 155:31–41

    Article  PubMed  CAS  Google Scholar 

  34. Castellani LW, Chang JJ, Wang X et al (2006) Transgenic mice express human MPO–463G/A alleles at atherosclerotic lesions, developing hyperlipidemia and obesity in –463G males. J Lipid Res 47:1366–1377

    Article  PubMed  CAS  Google Scholar 

  35. Leininger-Muller B, Hoy A, Herbeth B et al (2003) Myeloperoxidase G-463A polymorphism and Alzheimer’s disease in the ApoEurope study. Neurosci Lett 349:95–98

    Article  PubMed  CAS  Google Scholar 

  36. Lin GG, Du H, Chen JG et al (2010) Association of XPD/ERCC2 G23591A and A35931C polymorphisms with skin lesion prevalence in a multiethnic, arseniasis-hyperendemic village exposed to indoor combustion of high arsenic coal. Arch Toxicol 84:17–24

    Article  PubMed  CAS  Google Scholar 

  37. Lin GF, Du H, Chen JG et al (2007) Glutathione S-transferases M1 and T1 polymorphisms and arsenic content in hair and urine in two ethnic clans exposed to indoor combustion of high arsenic coal in Southwest Guizhou, China. Arch Toxicol 81:545–551

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was financed by grants from the China National Natural Scientific Foundation (81160335), the Foundation in the Ministry of Science and Technology of China (2010DFB30530 and 2011BAZ03220), and the Government Foundations in Guizhou Province of China [G(2011)7014 and S201104].

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Correspondence to Zhi-Zhong Guan.

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Zhang, T., Shan, KR., Tu, X. et al. Myeloperoxidase Activity and Its Corresponding mRNA Expression as well as Gene Polymorphism in the Population Living in the Coal-Burning Endemic Fluorosis Area in Guizhou of China. Biol Trace Elem Res 152, 379–386 (2013). https://doi.org/10.1007/s12011-013-9632-9

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  • DOI: https://doi.org/10.1007/s12011-013-9632-9

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