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Comparison Between 5-Aminosalicylic Acid (5-ASA) and Para-Aminosalicylic Acid (4-PAS) as Potential Protectors Against Mn-Induced Neurotoxicity

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Abstract

Manganese (Mn) is an essential metal for biological systems; however, occupational or clinical exposure to high levels of Mn can produce a neurological disorder called manganism. Oxidative stress and neuroinflammation play major roles in the Mn-induced neurodegeneration leading to dysfunction of the basal ganglia. We investigated the toxic effects of MnCl2 in an immortalized rat brain endothelial cell line (RBE4) and the protective effects of the radical scavenging aminosalicylic acids, 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic acid (4-PAS). Mn cytotoxicity was determined with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) activity. A significant decrease in MTT reduction concomitant with increased LDH release was noted in RBE4 cells exposed for 24 h to MnCl2 (600 and 800 μM; p < 0.0001). Our results establish that compared to 4-PAS, 5-ASA has greater efficacy in protecting RBE4 cells from Mn-induced neurotoxicity after preexposure to MnCl2 800 μM (p < 0.0001).

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Acknowledgments

I am thankful to Prof. Eunsook Lee from Meharry Medical College and Dr. Yingchun Yu from Vanderbilt University. The study was supported by FCT (Foundation for Science and Technology of Portugal; SFRH/BD/64128/2009) and the National Institute of Environmental Health Sciences R01 ES 10563 to MA.

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Authors and Affiliations

  1. I-Med. UL, Department of Toxicology and Food Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal

    Dinamene Santos, M. Camila Batoreu & Ana P. Marreilha dos Santos

  2. Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, 37232, USA

    Michael Aschner

Authors
  1. Dinamene Santos
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  2. M. Camila Batoreu
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  3. Michael Aschner
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  4. Ana P. Marreilha dos Santos
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Correspondence to Ana P. Marreilha dos Santos.

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Santos, D., Batoreu, M.C., Aschner, M. et al. Comparison Between 5-Aminosalicylic Acid (5-ASA) and Para-Aminosalicylic Acid (4-PAS) as Potential Protectors Against Mn-Induced Neurotoxicity. Biol Trace Elem Res 152, 113–116 (2013). https://doi.org/10.1007/s12011-012-9597-0

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  • DOI: https://doi.org/10.1007/s12011-012-9597-0

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