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Effect of Zinc Sulfate Fortificant on Iron Absorption from Low Extraction Wheat Flour Co-Fortified with Ferrous Sulfate

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Abstract

The co-fortification of wheat flour with iron (Fe) and zinc (Zn) is a strategy used to prevent these deficiencies in the population. Given that Zn could interact negatively with Fe, the objective was to assess the effect of Zn on Fe absorption from bread prepared with wheat flour fortified with Fe and graded levels of Zn fortificant. Twelve women aged 30–43 years, with contraception and a negative pregnancy test, participated in the study. They received on four different days, after an overnight fast, 100 g of bread made with wheat flour (70 % extraction) fortified with 30 mg Fe/kg as ferrous sulfate (A) or prepared with the same Fe-fortified flour but with graded levels of Zn, as zinc sulfate: 30 mg/kg (B), 60 mg/kg (C), or 90 mg/kg (D). Fe radioisotopes (59Fe and 55Fe) of high specific activity were used as tracers and Fe absorption iron was measured by the incorporation of radioactive Fe into erythrocytes. Results: The geometric mean and range of ±1 SD of Fe absorption were: A = 19.8 % (10.5–37.2 %), B = 18.5 % (10.2–33.4 %), C = 17.7 % (7.7–38.7 %), and D = 11.2 % (6.2–20.3 %), respectively; ANOVA for repeated measures F = 5.14, p < 0.01 (Scheffè’s post hoc test: A vs D and B vs D, p < 0.05). We can conclude that Fe is well absorbed from low extraction flour fortified with 30 mg/kg of Fe, as ferrous sulfate, and up to 60 mg/kg of Zn, as Zn sulfate. A statistically significant reduction of Fe absorption was observed at a Zn fortification level of 90 mg Zn/kg.

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Acknowledgments

The study was supported by grant 1100094 from Fondo Nacional de Ciencia y Tecnología Chile. The authors would like to express their gratitude to all the women who participated in the study and to Ms. Angelica Letelier for her technical assistance.

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Correspondence to Manuel Olivares.

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Olivares, M., Pizarro, F. & López de Romaña, D. Effect of Zinc Sulfate Fortificant on Iron Absorption from Low Extraction Wheat Flour Co-Fortified with Ferrous Sulfate. Biol Trace Elem Res 151, 471–475 (2013). https://doi.org/10.1007/s12011-012-9582-7

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  • DOI: https://doi.org/10.1007/s12011-012-9582-7

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