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The Effects of Mn2+ on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells

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Abstract

The effects of Mn2+ on the proliferation, osteogenic and adipogenic differentiation of BMSCs were evaluated by employing MTT, ΔΨm, cell cycle, ALP activity, collagen production, ARS and oil red O stain assays. The results indicated that Mn2+ decreased the viability at most concentrations for 24 h, but the viability was increased with prolonging incubation time. Mn2+ at the concentrations of 1 × 10-7 and 1 × 10-6 mol/L decreased ΔΨm in the BMSCs for 48 h. Mn2+ induced G2/M phase cell cycle arrest at tested concentrations. On day 7 and 10, the effect of Mn2+ on the osteogenic differentiation depended on concentration, but it inhibited osteogenic differentiation at all tested concentrations for 14 d. The effect of Mn2+ on the synthesis of collagen of BMSCs depended on concentration for 7 d, but Mn2+ inhibited the synthesis of collagen at all tested concentrations for 10 d. On day 14, Mn2+ inhibited the formation of mineralized matrix nodules of BMSCs at all tested concentrations, the inhibitory effect turned to be weaker with prolonging incubation time. Mn2+ promoted the adipogenic differentiation of BMSCs at all tested concentrations for 10 d, but had no effect with prolonging incubation time. These findings suggested the effects of Mn2+ on the proliferation, osteogenic differentiation and adipogenic differentiation of BMSCs are very complicated, concentration and incubation time are key factors for switching the biological effects of Mn2+ from damage to protection.

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Abbreviations

ALP:

Alkaline phosphatase

ARS:

Alizarin red S

BMSCs:

Bone marrow stromal cells

BSP:

Bone sialo protein

DMSO:

Dimethylsulfoxide

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

DMEM:

Dulbecco’s modified eagle’s medium

DLS:

Dynamic light scattering

KM:

Kun ming

ΔΨm:

Mitochondrial membrane potential

NBS:

Neonatal bovine serum

OD:

Optical density

OBs:

Osteoblasts

OS:

Osteogenic induction supplement

PTH:

Parathyroid hormone

PBS:

Phosphate buffer solution

PI:

Propidium iodide

Rh123:

Rhodamine 123

SD:

Standard deviation

References

  1. Samelson EJ, Hannan MT (2006) Epidemiology of osteoporosis. Curr Rheumatol Rep 1:76–83

    Article  Google Scholar 

  2. Hodges SJ, Pilkington M, Stamp TC et al (1991) Depressed levels of circulating menaquinones in patients with osteoporotic fractures of the spine and femoral neck. Bone 12:387–389

    Article  PubMed  CAS  Google Scholar 

  3. Relea P, Revilla M, Ripoll E et al (1995) Zinc, biochemical markers of nutrition, and type I osteoporosis. Age Ageing 24:303–307

    Article  PubMed  CAS  Google Scholar 

  4. Dawson-Hughes B, Harris SS, Krall EA et al (1997) Effect of calcium and vitamin D supplementation on bone density in men and women 65 years of age or older. N Engl J Med 337:670–676

    Article  PubMed  CAS  Google Scholar 

  5. Munger RG, Cerhan JR, Chiu BCH (1999) Prospective study of protein intake and risk of hip fracture in postmenopausal women. Am J Clin Nutr 69:147–152

    PubMed  CAS  Google Scholar 

  6. Strause L, Saltman P, Smith KT et al (1994) Spinal bone loss in postmenopausal women supplemented with calcium and trace minerals. J Nutr 124:1060–1064

    PubMed  CAS  Google Scholar 

  7. Rico H, Gómez-Raso N, Revilla M et al (2000) Effects on bone loss of manganese alone or with copper supplement in ovariectomized rats. A morphometric and densitomeric study. Eur J Obstet Gynecol Reprod Biol 90:97–101

    Article  PubMed  CAS  Google Scholar 

  8. Bae YJ, Kim MH (2008) Manganese supplementation improves mineral density of the spine and femur and serum osteocalcin in rats. Biol Trace Elem Res 124:28–34

    Article  PubMed  CAS  Google Scholar 

  9. Odabasi E, Turan M, Aydin A et al (2008) Magnesium, zinc, copper, manganese, and selenium levels in postmenopausal women with osteoporosis. Can magnesium play a key role in osteoporosis? Ann Acad Med Singapore 37:564–567

    PubMed  Google Scholar 

  10. Stern PH (1985) Biphasic effects of manganese on hormone-stimulated bone resorption. Endocrinology 117(5):2044–2049

    Article  PubMed  CAS  Google Scholar 

  11. Ahdjoudj S, Fromigue O, Marie PJ (2004) Plasticity and regulation of human bone marrow stromal osteoprogenitor cells: potential implication in the treatment of age-related bone loss. Histol Histopathol 19:151–157

    PubMed  CAS  Google Scholar 

  12. Nuttall ME, Gimble JM (2000) Is there a therapeutic opportunity to either prevent or treat osteopenic disorders by inhibiting marrow adipogenesis? Bone 27:177–184

    Article  PubMed  CAS  Google Scholar 

  13. Ailhaud G, Grimaldi P, Negrel R (1992) Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 12:207–233

    Article  PubMed  CAS  Google Scholar 

  14. Verma S, Rajaratnam JH, Denton J et al (2002) Adipoctyic proportion of bone marrow is inversely related to bone formation in osteoporosis. J Clin Pathol 55:693–698

    Article  PubMed  CAS  Google Scholar 

  15. Lüthen F, Bulnheim U, Müller PD et al (2007) Influence of manganese ions on cellular behavior of human osteoblasts in vitro. Biomol Eng 24:531–536

    Article  PubMed  Google Scholar 

  16. Lampugnani MG, Barnasconi S, Neri P et al (1991) Role of manganese in MG-63 ostosarcoma cell attachment to fibrinogen and von Willebrand factor. Lab Invest 65:96–103

    PubMed  CAS  Google Scholar 

  17. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  PubMed  CAS  Google Scholar 

  18. Zhao WH, Gou BD, Zhang TL et al (2012) Lanthanum chloride bidirectionally influences calcification in bovine vascular smooth muscle cells. J Cell Biochem 113:1776–1786

    PubMed  CAS  Google Scholar 

  19. Nabanita SD, Chen C, Janice EB et al (2005) PTHrP signaling targets cyclin D1 and induces osteoblastic cell growth arrest. J Bone Miner Res 20:1051–1064

    Article  Google Scholar 

  20. Ferrer EG, Salinas MV, Correa MJ et al (2006) Synthesis, characterization, antitumoral and osteogenic activities of quercetin vanadyl (IV) complexes. J Biol Inorg Chem 11:791–801

    Article  PubMed  CAS  Google Scholar 

  21. Gori F, Divieti P, Demay MB (2001) Cloning and characterization of a novel WD-40 repeat protein that dramatically accelerates osteoblastic differentiation. J Biol Chem 276:46515–46522

    Article  PubMed  CAS  Google Scholar 

  22. Sekiya I, Larson BL, Vuoristo JT et al (2004) Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs). J Bone Miner Res 19:256–264

    Article  PubMed  CAS  Google Scholar 

  23. Sakaguchi K, Morita I, Murota S (2000) Relationship between the ability to support differentiation of osteoclast-like cells and adipogenesis in murine stromal cells derived from bone marrow. Prostage Leuk Otr Ess 62:319–327

    Article  CAS  Google Scholar 

  24. Ozturk SS, Hu WS (2006) Cell culture technology for pharma-ceutical and cell-based therapies. CRC Press, Boca Raton

    Google Scholar 

  25. Li JX, Liu JC, Wang K et al (2010) Gadolinium-containing bioparticles as an active entity to promote cell cycle progression in mouse embryo fibroblast NIH3T3 cells. J Biol Inorg Chem 15:547–557

    Article  PubMed  CAS  Google Scholar 

  26. Tim S, Katherine H, Thompsona et al (2006) Design of targeting ligands in medicinal inorganic chemistry. Chem Soc Rev 35:534–544

    Article  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 20971034) and the Doctoral Program Foundation of Institutions of Higher Education of China (No. 20111301110004).

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Authors and Affiliations

  1. College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People’s Republic of China

    Jinchao Zhang, Qun Zhang & Shenghui Li

  2. College of Basic Medicine, Hebei University, Baoding, People’s Republic of China

    Yingjian Hou

  3. Affiliated Hospital of Hebei University, Baoding, People’s Republic of China

    Haisong Zhang

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  1. Jinchao Zhang
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  2. Qun Zhang
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  3. Shenghui Li
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Correspondence to Jinchao Zhang.

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Zhang, J., Zhang, Q., Li, S. et al. The Effects of Mn2+ on the Proliferation, Osteogenic Differentiation and Adipogenic Differentiation of Primary Mouse Bone Marrow Stromal Cells. Biol Trace Elem Res 151, 415–423 (2013). https://doi.org/10.1007/s12011-012-9581-8

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  • DOI: https://doi.org/10.1007/s12011-012-9581-8

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