Biological Trace Element Research

, Volume 150, Issue 1, pp 328–336

Evaluating the Potential Role of Pomegranate Peel in Aluminum-Induced Oxidative Stress and Histopathological Alterations in Brain of Female Rats


DOI: 10.1007/s12011-012-9498-2

Cite this article as:
Abdel Moneim, A.E. Biol Trace Elem Res (2012) 150: 328. doi:10.1007/s12011-012-9498-2


Studies have shown that pomegranate, Punica granatum Linn. (Lythraceae), has remarkable biological and medicinal properties. However, the effects of pomegranate peel methanolic extract (PPME) on the aluminum-induced oxidative stress and histopathological change have not been reported yet. To determine the effect of PPME (200 mg/kg bwt) on the aluminum chloride (AlCl3; 34 mg/kg bwt)-induced neurotoxicity, aluminum accumulation in brain and oxidant/antioxidant status were determined. The change of brain structure was investigated with hematoxylin and eosin, and anti-apoptosis effects of PPME were analyzed by immunohistochemistry. The present study showed an indication of carcinogenicity in the AlCl3-treated group representing an increase in tissue tumor markers such as tumor necrosis factor-α and angiogenin and inflammation by inducing an increase in prostaglandin E2 and prostaglandin F2α. PPME protected brain through decreasing the aluminum accumulation and stimulating antioxidant activities and anti-apoptotic proteins namely Bcl-2. Therefore, these results indicated that pomegranate peel methanolic extract could inhibit aluminum-induced oxidative stress and histopathological alternations in brain of female rats, and these effects may be related to anti-apoptotic and antioxidants activities.


Aluminum chloridePomegranate peelOxidant/antioxidant statusAnti-apoptosisBrainRats

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Biomedical Research Center, Health Sciences Technology ParkUniversity of GranadaGranadaSpain
  2. 2.Department of Zoology and Entomology, Faculty of ScienceHelwan UniversityCairoEgypt