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Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification

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Abstract

Wheat straw was pretreated by phosphoric acid plus hydrogen peroxide (PHP), in which temperature, time, and H3PO4 proportion for pretreatment were investigated by using response surface method. Results indicated that hemicellulose and lignin removal positively responded to the increase of pretreatment temperature, H3PO4 proportion, and time. H3PO4 proportion was the most important variable to control cellulose recovery, followed by pretreatment temperature and time. Moreover, these three variables all negatively related to cellulose recovery. Increasing H3PO4 proportion can improve enzymatic hydrolysis; however, reduction on cellulose recovery results in decrease of glucose yield. Extra high temperature or long time for pretreatment was not beneficial to enzymatic hydrolysis and glucose yield. Based on the criterion for minimizing H3PO4 usage and maximizing glucose yield, the optimized pretreatment conditions was 40 °C, 2.0 h, and H3PO4 proportion of 70.2 % (H2O2 proportion of 5.2 %), by which glucose yielded 299 mg/g wheat straw (946.2 mg/g cellulose) after 72-h enzymatic hydrolysis.

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Acknowledgments

This work is supported by National Natural Science Foundation of China (21306120) and the Program for “Changjiang Scholars and Innovative Research Team in University” (IRT13083) from the Ministry and Education of China. The Department of Science and Technology of Sichuan Province is also appreciated for the funding supports (nos. 2014JQ0037 and 2015NZ0100).

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  1. Fei Shen

    Present address: Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People’s Republic of China

Authors and Affiliations

  1. Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu, Sichuan, 611130, People’s Republic of China

    Jingwen Qiu, Qing Wang, Gang Yang, Yanzong Zhang, Shihuai Deng, Jing Zhang, Yongmei Zeng & Chun Song

  2. Department of Information Consultation and Integration, Library of Sichuan Agricultural University, Chengdu, Sichuan, 611130, People’s Republic of China

    Yongmei Zeng

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  1. Jingwen Qiu
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Correspondence to Fei Shen.

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Qiu, J., Wang, Q., Shen, F. et al. Optimizing Phosphoric Acid plus Hydrogen Peroxide (PHP) Pretreatment on Wheat Straw by Response Surface Method for Enzymatic Saccharification. Appl Biochem Biotechnol 181, 1123–1139 (2017). https://doi.org/10.1007/s12010-016-2273-7

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  • DOI: https://doi.org/10.1007/s12010-016-2273-7

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