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Evaluation of the Fermentation Potential of Pulp Mill Residue to Produce d(−)-Lactic Acid by Separate Hydrolysis and Fermentation Using Lactobacillus coryniformis subsp. torquens

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Abstract

Lactic acid is widely used in chemical, pharmaceutical, cosmetic, and food industries, besides it is the building block to produce polylactic acid, which is a sustainable alternative biopolymer to synthetic plastic due to its biodegradability. Aiming at producing an optically pure isomer, the present work evaluated the potential of pulp mill residue as feedstock to produce d(−)-lactic acid by a strain of the bacterium Lactobacillus coryniformis subsp. torquens using separate hydrolysis and fermentation process. Enzymatic hydrolysis, optimized through response surface methodology for 1 g:4 mL solid/liquid ratio and 24.8 FPU/gcellulose enzyme loading, resulted in 140 g L−1 total reducing sugar and 110 g L−1 glucose after 48 h, leading to 61 % of efficiency. In instrumented bioreactor, 57 g L−1 of d(−)-lactic acid was achieved in 20 h of fermentation, while only 0.5 g L−1 of l(+)-lactic acid was generated. Furthermore, product yield of 0.97 g/g and volumetric productivity of 2.8 g L−1 h−1 were obtained.

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Acknowledgments

The authors would like to thank the colleagues of LADEBIO/EQ/UFRJ for all technical support during the experiments and the AGIR/UFF for PIBITI scholarship.

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Authors and Affiliations

  1. Departamento de Engenharia Química e Petróleo, Universidade Federal Fluminense, Rua Passo da Pátria, 156—Campus da Praia Vermelha, São Domingos, Niterói, RJ, 24210-310, Brazil

    Anelize de Oliveira Moraes & Ninoska Isabel Bojorge Ramirez

  2. Centro de Tecnologia, Escola de Química, Departamento de Engenharia Bioquímica, Universidade Federal do Rio de Janeiro, Avenida Athos da Silveira Ramos, 149—Cidade Universitária, Rio de Janeiro, RJ, 21941-909, Brazil

    Nei Pereira Jr

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  1. Anelize de Oliveira Moraes
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  2. Ninoska Isabel Bojorge Ramirez
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  3. Nei Pereira Jr
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Correspondence to Nei Pereira Jr.

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de Oliveira Moraes, A., Ramirez, N.I.B. & Pereira, N. Evaluation of the Fermentation Potential of Pulp Mill Residue to Produce d(−)-Lactic Acid by Separate Hydrolysis and Fermentation Using Lactobacillus coryniformis subsp. torquens . Appl Biochem Biotechnol 180, 1574–1585 (2016). https://doi.org/10.1007/s12010-016-2188-3

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  • DOI: https://doi.org/10.1007/s12010-016-2188-3

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