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Wet and Dry Forms of Bacterial Cellulose Synthetized by Different Strains of Gluconacetobacter xylinus as Carriers for Yeast Immobilization

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Abstract

The present study aimed to explore and describe the properties of bacterial cellulose (BC) membranes obtained from three different strains of Gluconacetobacter xylinus for 72, 120, and 168 h, used as a carrier support for the immobilization of Saccharomyces cerevisiae. The experiments also included the analysis of glucose consumption and alcohol production during the fermentation process displayed by yeasts immobilized on the BC surface. The results of the present study demonstrate that the number of immobilized yeast cells is dependent on the type of cellulose-synthesizing strain, cellulose form, and duration of its synthesis. The BC in the form of wet membranes obtained after 3 days of synthesis displayed the most favorable properties as a carrier for yeast immobilization. The immobilization of yeast cells on BC, regardless of its form, increased the amount of the produced alcohol as compared to free cells. The yeast cells immobilized in BC were able to multiply on its surface during the fermentation process.

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Acknowledgments

This work was supported by the National Centre for Research and Development in Poland (Grant No. LIDER/011/221/L-5/13/NCBR/2014).

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Authors and Affiliations

  1. Department of Immunology, Microbiology and Physiological Chemistry, West Pomeranian University of Technology, Szczecin, Piastów 45, 70-311, Szczecin, Poland

    Anna Żywicka, Dorota Peitler & Karol Fijałkowski

  2. Institute of Chemical Engineering and Environmental Protection Processes, West Pomeranian University of Technology, Szczecin, Piastów 42, 71-065, Szczecin, Poland

    Rafał Rakoczy

  3. Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, Borowska 211a, 50-556, Wroclaw, Poland

    Adam Feliks Junka

Authors
  1. Anna Żywicka
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  2. Dorota Peitler
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  3. Rafał Rakoczy
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  4. Adam Feliks Junka
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  5. Karol Fijałkowski
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Correspondence to Karol Fijałkowski.

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Żywicka, A., Peitler, D., Rakoczy, R. et al. Wet and Dry Forms of Bacterial Cellulose Synthetized by Different Strains of Gluconacetobacter xylinus as Carriers for Yeast Immobilization. Appl Biochem Biotechnol 180, 805–816 (2016). https://doi.org/10.1007/s12010-016-2134-4

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  • DOI: https://doi.org/10.1007/s12010-016-2134-4

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