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Enhancement of Cellulase and Xylanase Production Using pH-Shift and Dissolved Oxygen Control Strategy with Streptomyces griseorubens JSD-1

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Abstract

In this study, the production of cellulase and xylanase by Streptomyces griseorubens JSD-1 was improved by integrating the pH-shift and dissolved oxygen (DO)-constant control strategies. The pH-shift control strategy was carried out by analyzing the specific cell growth rate (μ) and specific enzyme formation rate (Q p) of S. griseorubens JSD-1. The pH was controlled at 8.0 during the first 48 h to maintain high cell growth, which then shifted to 7.5 after 48 h to improve the production of cellulase and xylanase. Using this method, the maximum activities of cellulase, xylanase, and filter paper enzyme (FPase) increased by 47.9, 29.5, and 113.6 %, respectively, compared to that obtained without pH control. On the basis of pH-shift control, the influence of DO concentrations on biomass and enzyme production was further investigated. The maximum production of cellulase, xylanase, and FPase reached 114.38 ± 0.96 U mL−1, 330.57 ± 2.54 U mL−1, and 40.11 ± 0.38 U mL−1, which were about 1.6-fold, 0.6-fold, and 3.2-fold higher than that of neutral pH without DO control conditions. These results supplied a functional approach for improving cellulase and xylanase production.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (2012AA101405) and the National Natural Science Foundation of China (31201682).

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Authors and Affiliations

  1. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of China

    Dan Zhang, Yanqing Luo, Shaohua Chu, Yuee Zhi, Bin Wang & Pei Zhou

  2. Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai Jiaotong University, No. 800 Dongchuan Road, Minhang District, Shanghai, 200240, People’s Republic of China

    Dan Zhang, Yanqing Luo, Shaohua Chu, Yuee Zhi, Bin Wang & Pei Zhou

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  1. Dan Zhang
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Correspondence to Pei Zhou.

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Zhang, D., Luo, Y., Chu, S. et al. Enhancement of Cellulase and Xylanase Production Using pH-Shift and Dissolved Oxygen Control Strategy with Streptomyces griseorubens JSD-1. Appl Biochem Biotechnol 178, 338–352 (2016). https://doi.org/10.1007/s12010-015-1875-9

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