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Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System

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Abstract

To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.25 and 100 μg/mL for both methods. The clenbuterol limit of detection for the target site compensation method and solution competition method are estimated to be 6.7 and 4.5 μg/mL, respectively. The proposed methods were successfully applied to the detection of clenbuterol molecules and were found to have high specificity and high-throughput and were label free and operationally convenient.

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Abbreviations

CLEN:

Clenbuterol

anti-CLEN antibody:

Anti-clenbuterol polyclonal antibody

PDMS:

Polydimethylsiloxane

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Acknowledgments

This work was supported by NSFC (Grant No. 81371642) and the Fundamental Research Funds for the Central Universities of China.

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Authors and Affiliations

  1. Functional Materials Research Laboratory, Tongji University, Shanghai, People’s Republic of China

    Yichuan Wu & Manwen Yao

  2. School of Science, Xi’an Jiaotong University, Xi’an, People’s Republic of China

    Xiangyi Fang

  3. Department of Clinical Laboratory, the First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an, People’s Republic of China

    Yucong Yang & Xiaoli Cheng

Authors
  1. Yichuan Wu
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  2. Manwen Yao
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  3. Xiangyi Fang
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  4. Yucong Yang
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  5. Xiaoli Cheng
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Correspondence to Xiangyi Fang.

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Wu, Y., Yao, M., Fang, X. et al. Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System. Appl Biochem Biotechnol 177, 1327–1337 (2015). https://doi.org/10.1007/s12010-015-1817-6

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  • DOI: https://doi.org/10.1007/s12010-015-1817-6

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