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Characterization and Differentiation of Stem Cells Isolated from Human Newborn Foreskin Tissue

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Abstract

Circumcision is described as a cultural, medical, and religious process which states surgical removal of the foreskin either partly or fully. Cells isolated from the circumcised tissues are referred as foreskin cells. They have been thought as feeder cell lines for embryonic stem cells. Their fibroblastic properties were also utilized for several experiments. The waste tissues that remain after the circumcision thought to have stem cell properties. Therefore, there have been very few attempts to expose their stem cell properties without turning them into induced pluripotent stem cells. Although stem cell isolation from prepuce and their mesenchymal multilineage differentiation potential have been presented many times in the literature, the current study explored hematopoietical phenotype of newborn foreskin stem cells for the first time. According to the results, human newborn foreskin stem cells (hnFSSCs) were identified by their capability to turn into all three germ layer cell types under in vitro conditions. In addition, these cells have exhibited a stable phenotype and have remained as a monolayer in vitro. hnFSSCs suggested to carry different treatment potentials for bone damages, cartilage problems, nerve damages, lesion formations, and other diseases that are derive from mesodermal, endodermal, and ectodermal origins. Owing to the location of the tissue in the body and differentiation capabilities of hnFSSCs, these cells can be considered as easily obtainable and utilizable even better than the other stem cell sources. In addition, hnFSSCs offers a great potential for tissue engineering approaches due to exhibiting embryonic stem cell-like characteristics, not having any ethical issues, and teratoma induction as in embryonic stem cell applications.

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Acknowledgments

We would like to thank Burçin Keskin for her help. The authors deny any conflicts of interest.

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Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, 26 Ağustos Campus, Kayisdagi Cad., Kayisdagi, 34755, Istanbul, Turkey

    Özge Sezin Somuncu, Pakize Neslihan Taşlı, Hatice Burcu Şişli & Fikrettin Şahin

  2. Department of Pediatric Surgery, School of Medicine, Bahçeşehir University, Istanbul, Turkey

    Salih Somuncu

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  1. Özge Sezin Somuncu
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  2. Pakize Neslihan Taşlı
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  3. Hatice Burcu Şişli
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Correspondence to Fikrettin Şahin.

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Supplementary Data

Mesenchymal stem cell characterization of human newborn foreskin stem cells at passage 3. Major population of cells maintained the characteristics of stem cells. Flow cytometry analysis data shows the positive mesenchymal stem cell surface markers CD29, CD44, CD73, and CD90 and the negative endothelial stem cell surface marker CD31. Data also indicates the positive hematopoietic stem cell surface markers CD45, CD34, and CD14. NC negative control. (GIF 293 kb)

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Somuncu, Ö.S., Taşlı, P.N., Şişli, H.B. et al. Characterization and Differentiation of Stem Cells Isolated from Human Newborn Foreskin Tissue. Appl Biochem Biotechnol 177, 1040–1054 (2015). https://doi.org/10.1007/s12010-015-1795-8

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  • DOI: https://doi.org/10.1007/s12010-015-1795-8

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