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Study of Biological Degradation of New Poly(Ether-Urethane-Urea)s Containing Cyclopeptide Moiety and PEG by Bacillus amyloliquefaciens Isolated from Soil

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Abstract

The present work for the first time investigates the effect of Bacillus amyloliquefaciens, M3, on a new poly(ether-urethane-urea) (PEUU). PEUU was synthesized via reaction of 4,4′-methylenebis(4-phenylisocyanate) (MDI), l-leucine anhydride cyclopeptide (LACP) as a degradable monomer and polyethylene glycol with molecular weight of 1000 (PEG-1000). Biodegradation of the synthesized PEUU as the only source for carbon and nitrogen for M3 was studied. The co-metabolism biodegradation of the polymer by this organism was also investigated by adding mannitol or nutrient broth to the basic media. Biodegradation of the synthesized polymer was followed by SEM, FT-IR, TGA, and XRD techniques. It was shown that incubation of PEUU with M3 resulted in a 30–44 % reduction in polymer’s weight after 1 month. This study indicates that the chemical structure of PEUU significantly changes after exposure to M3 due to hydrolytic and enzymatic degradation of polymer chains. The results of this work supports the idea that this poly(ether-urethane) is used as a sole carbon source by M3 and this bacterium has a good capability for degradation of poly(ether-urethane)s.

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Acknowledgments

The financial support of this work by Research Affairs Division University of Isfahan (UI) is gratefully acknowledged. We also extend our special thanks to Miss Shahrzad Dehghanfar, M.S. student of Department of Biology, Microbiology Division, and Dr. Delaram Fallahi for the useful help.

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Authors and Affiliations

  1. Polymer Chemistry Research Laboratory, Department of Chemistry, University of Isfahan, Isfahan, 81746-73441, Islamic Republic of Iran

    Fatemeh Rafiemanzelat & Mahboobeh Jafari

  2. Department of Biology, Faculty of Sciences, University of Isfahan, P.O. Box 117, Isfahan, Islamic Republic of Iran

    Giti Emtiazi

Authors
  1. Fatemeh Rafiemanzelat
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  2. Mahboobeh Jafari
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  3. Giti Emtiazi
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Correspondence to Fatemeh Rafiemanzelat or Giti Emtiazi.

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Rafiemanzelat, F., Jafari, M. & Emtiazi, G. Study of Biological Degradation of New Poly(Ether-Urethane-Urea)s Containing Cyclopeptide Moiety and PEG by Bacillus amyloliquefaciens Isolated from Soil. Appl Biochem Biotechnol 177, 842–860 (2015). https://doi.org/10.1007/s12010-015-1782-0

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