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Probing of the Interaction Between β-Lactoglobulin and the Anticancer Drug Oxaliplatin

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Abstract

The potential carrier role of β-lactoglobulin (β-LG) and its interactions with oxaliplatin were studied using various spectroscopic techniques (fluorescence, UV–visible, and circular dichroism (CD)) in an aqueous medium at two temperatures of 25 and 37 °C in combination with a molecular docking study. Fluorescence measurements have shown that the observed quenching is a combination of static and dynamic quenching with a predominant contribution of static mode. The presence of a single binding site located in the internal cavity of the β-barrel of β-LG was confirmed by molecular docking calculations. Thermodynamic data as well as molecular docking indicated that the hydrophobic interactions dominate in the binding site. Results of fluorescence resonance energy transfer (FRET) measurements in combination with docking results imply that resonance energy transfer occurs between β-LG and its ligand oxaliplatin. Additionally, CD results revealed that oxaliplatin binding has no influence on the β-LG structure. The molecular docking results indicate that docking may be an appropriate method for the prediction and confirmation of experimental results. Complementary molecular docking results may be useful for the determination of the binding mechanism of proteins such as β-LG in pharmaceutical and biophysical studies providing new insight in the novel pharmacology and new solutions in the formulation of advanced oral drug delivery systems.

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Abbreviations

β-LG:

β-Lactoglobulin

CD:

Circular dichroism

FRET:

Fluorescence resonance energy transfer

ANS:

8-Anilino-1-naphthalenesulfonate

MRW:

Mean amino acid residue weight

GA:

Genetic algorithm

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Behafarid Ghalandari & Kazem Parivar

  2. Department of Biological Sciences, Kharazmi University, Tehran, Iran

    Adeleh Divsalar

  3. Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran

    Mahbube Eslami-Moghadam

  4. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

  5. Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

  6. Fonctions et Interactions des Protéines, Biopolymères, Interactions, Associations, Institut National de la Recherche Agronomique, Nantes, France

    Thomas Haertlé

  7. Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Massoud Amanlou

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  1. Behafarid Ghalandari
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  2. Adeleh Divsalar
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Correspondence to Behafarid Ghalandari or Adeleh Divsalar.

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Ghalandari, B., Divsalar, A., Eslami-Moghadam, M. et al. Probing of the Interaction Between β-Lactoglobulin and the Anticancer Drug Oxaliplatin. Appl Biochem Biotechnol 175, 974–987 (2015). https://doi.org/10.1007/s12010-014-1341-0

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  • DOI: https://doi.org/10.1007/s12010-014-1341-0

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