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High Polymorphism in Est-SSR Loci for Cellulose Synthase and β-Amylase of Sugarcane Varieties (Saccharum spp.) Used by the Industrial Sector for Ethanol Production

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Abstract

High and low polymorphisms in simple sequence repeats of expressed sequence tag (EST-SSR) for specific proteins and enzymes, such as β-amylase, cellulose synthase, xyloglucan endotransglucosylase, fructose 1,6-bisphosphate aldolase, and fructose 1,6-bisphosphatase, were used to illustrate the genetic divergence within and between varieties of sugarcane (Saccharum spp.) and to guide the technological paths to optimize ethanol production from lignocellulose biomass. The varieties RB72454, RB867515, RB92579, and SP813250 on the second stage of cutting, all grown in the state of Paraná (PR), and the varieties RB92579 and SP813250 cultured in the PR state and in Northeastern Brazil, state of Pernambuco (PE), were analyzed using five EST-SSR primers for EstC66, EstC67, EstC68, EstC69, and EstC91 loci. Genetic divergence was evident in the EstC67 and EstC69 loci for β-amylase and cellulose synthase, respectively, among the four sugarcane varieties. An extremely high level of genetic differentiation was also detected in the EstC67 locus from the RB82579 and SP813250 varieties cultured in the PR and PE states. High polymorphism in SSR of the cellulose synthase locus may explain the high variability of substrates used in pretreatment and enzymatic hydrolysis processes, which has been an obstacle to effective industrial adaptations.

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Acknowledgments

The authors would like to thank CNPq (Conselho Nacional de Pesquisa Ciência e Tecnologia, Brasília, DF), Fundação Araucária (Curitiba, PR), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasília DF Brazil) for their financial support. We would also like to thank Fazenda Junqueira (Alto Alegre, Colorado, PR, Brazil) for the samples provided.

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Authors and Affiliations

  1. Postgraduate Program in Genetics and Breeding, State University of Maringá, Maringá, Paraná, 87020-900, Brazil

    Raphael Augusto

  2. Postgraduate Program in Agronomy, State University of Maringá, Maringá, Paraná, 87020-900, Brazil

    Rone Charles Maranho

  3. Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá, Paraná, 87020-900, Brazil

    Claudete Aparecida Mangolin & Maria de Fátima Pires da Silva Machado

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  1. Raphael Augusto
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  2. Rone Charles Maranho
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  3. Claudete Aparecida Mangolin
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  4. Maria de Fátima Pires da Silva Machado
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Correspondence to Maria de Fátima Pires da Silva Machado.

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Augusto, R., Maranho, R.C., Mangolin, C.A. et al. High Polymorphism in Est-SSR Loci for Cellulose Synthase and β-Amylase of Sugarcane Varieties (Saccharum spp.) Used by the Industrial Sector for Ethanol Production. Appl Biochem Biotechnol 175, 965–973 (2015). https://doi.org/10.1007/s12010-014-1340-1

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