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Isolation and Characterization of Poly(3-hydroxybutyrate)-Producing Bacteria from Aerobic Sludge

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Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable and environmentally friendly natural polymers. In this study, we isolated a bacterium strain capable of synthesizing PHAs from the aerobic sludge of a sewage treatment plant. The bacterium was identified as Burkholderia cepacia via physiological and biochemical tests as well as 16S rDNA sequence analysis. Strain WN-H41 produced PHAs, which was identified as P3HB. These PHAs have a number average molecular weight of 2.6 × 104 Da, a polydispersity index (PDI) of 2.4, and its thermal properties include a glass transition temperature of 1 °C, a melting temperature of 171 °C, and a decomposition temperature of 280 °C. These properties indicate that P3HB produced by WN-H41 has a high purity and good thermal stability.

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Acknowledgments

The authors are grateful to the school of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, China for providing all the laboratory facilities.

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Authors and Affiliations

  1. Department of Environmental and Biological Engineering, School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832000, China

    Bingxin Zheng, Jianjiang Lu, Yanbin Tong, Hongling Li & Qianqian Chen

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  1. Bingxin Zheng
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  2. Jianjiang Lu
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  3. Yanbin Tong
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  4. Hongling Li
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  5. Qianqian Chen
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Correspondence to Jianjiang Lu.

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Zheng, B., Lu, J., Tong, Y. et al. Isolation and Characterization of Poly(3-hydroxybutyrate)-Producing Bacteria from Aerobic Sludge. Appl Biochem Biotechnol 175, 421–427 (2015). https://doi.org/10.1007/s12010-014-1271-x

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  • DOI: https://doi.org/10.1007/s12010-014-1271-x

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