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The Effect of Nonenzymatic Protein on Lignocellulose Enzymatic Hydrolysis and Simultaneous Saccharification and Fermentation

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Abstract

Nonenzymatic protein was added to cellulase hydrolysis and simultaneous saccharification and fermentation (SSF) of different biomass materials. Adding bovine serum albumin (BSA) and corn steep before cellulase enhanced enzyme activity in solution and increased cellulose and xylose conversion rates. The cellulose conversion rate of filter paper hydrolysis was increased by 32.5 % with BSA treatment. When BSA was added before cellulase, the remaining activity in the solution was higher than that in a control without BSA pretreatment. During SSF with pretreated rice straw as the substrate, adding 1.0 mg/mL BSA increased the ethanol yield by 13.6 % and final xylose yield by 42.6 %. The results indicated that lignin interaction is not the only mechanism responsible for the positive BSA effect. BSA had a stabilizing effect on cellulase and relieved cumulative sugar inhibition of enzymatic hydrolysis of biomass materials. Thus, nonenzymatic protein addition represents a promising strategy in the biorefining of lignocellulose materials.

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Acknowledgments

This work was supported by JST/JICA-SATREPS, “Sustainable Integration of Local Agriculture and Biomass Industries,” and a grant from the National High Technology Research and Development Program of China (863 Program) (No. 2012AA101803). The English language was reviewed by Enago (www.Enago.jp).

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Authors and Affiliations

  1. Institute of Industrial Science, The University of Tokyo, Tokyo, 153-8505, Japan

    Hui Wang, Shinichi Kobayashi, Hatsue Hiraide & Kazuhiro Mochidzuki

  2. College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing, 100193, China

    Zongjun Cui

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  1. Hui Wang
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  2. Shinichi Kobayashi
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  3. Hatsue Hiraide
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  4. Zongjun Cui
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  5. Kazuhiro Mochidzuki
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Correspondence to Kazuhiro Mochidzuki.

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Wang, H., Kobayashi, S., Hiraide, H. et al. The Effect of Nonenzymatic Protein on Lignocellulose Enzymatic Hydrolysis and Simultaneous Saccharification and Fermentation. Appl Biochem Biotechnol 175, 287–299 (2015). https://doi.org/10.1007/s12010-014-1242-2

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  • DOI: https://doi.org/10.1007/s12010-014-1242-2

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