Applied Biochemistry and Biotechnology

, Volume 174, Issue 8, pp 2864–2874

Purification and Characterization of Recombinant Cel7A from Maize Seed

  • Nathan C. Hood
  • Kendall R. Hood
  • Susan L. Woodard
  • Shivakumar P. Devaiah
  • Tina Jeoh
  • Lisa Wilken
  • Zivko Nikolov
  • Erin Egelkrout
  • John A. Howard
  • Elizabeth E. Hood
Article

DOI: 10.1007/s12010-014-1232-4

Cite this article as:
Hood, N.C., Hood, K.R., Woodard, S.L. et al. Appl Biochem Biotechnol (2014) 174: 2864. doi:10.1007/s12010-014-1232-4

Abstract

The corn grain biofactory was used to produce Cel7A, an exo-cellulase (cellobiohydrolase I) from Hypocrea jecorina. The enzymatic activity on small molecule substrates was equivalent to its fungal counterpart. The corn grain-derived enzyme is glycosylated and 6 kDa smaller than the native fungal protein, likely due to more sugars added in the glycosylation of the fungal enzyme. Our data suggest that corn seed-derived cellobiohydrolase (CBH) I performs as well as or better than its fungal counterpart in releasing sugars from complex substrates such as pre-treated corn stover or wood. This recombinant protein product can enter and expand current reagent enzyme markets as well as create new markets in textile or pulp processing. The purified protein is now available commercially.

Keywords

Cel7ARecombinant proteinCellobiohydrolase IProtein purificationCellulaseBiomass conversion

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Nathan C. Hood
    • 1
  • Kendall R. Hood
    • 1
  • Susan L. Woodard
    • 2
  • Shivakumar P. Devaiah
    • 3
  • Tina Jeoh
    • 4
  • Lisa Wilken
    • 5
  • Zivko Nikolov
    • 6
  • Erin Egelkrout
    • 7
  • John A. Howard
    • 7
  • Elizabeth E. Hood
    • 1
  1. 1.Infinite Enzymes, LLCState UniversityUSA
  2. 2.Kalon BiotherapeuticsCollege StationUSA
  3. 3.Department of Biological SciencesEast Tennessee State UniversityJohnson CityUSA
  4. 4.Department of Biological and Agricultural EngineeringUniversity of CaliforniaDavisUSA
  5. 5.Biological and Agricultural EngineeringKansas State UniversityManhattanUSA
  6. 6.Biological and Agricultural EngineeringTexas A&M UniversityCollege StationUSA
  7. 7.Applied Biotechnology Institute, Cal Poly Tech ParkSan Luis ObispoUSA