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Alginate/Polyoxyethylene and Alginate/Gelatin Hydrogels: Preparation, Characterization, and Application in Tissue Engineering

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Abstract

Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxyethylene as a supporting material. In an alginate/PEO sample that contains 20 % polyoxyethylene, we obtained a stable hydrogel for cell culture experiments. We also prepared a stable alginate/gelatin hydrogel by cross-linking a periodate-oxidized alginate with another functional component such as gelatin. The hydrogels were found to have a high fluid uptake. In this work, preparation, characterization, swelling, and surface properties of these scaffold materials were described. Lyophilized scaffolds obtained from hydrogels were used for cell viability experiments, and the results were presented in detail.

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Authors and Affiliations

  1. Department of Chemistry, Engineering Faculty, Istanbul University, 34320, Avcilar-Istanbul, Turkey

    Ayse Z. Aroguz

  2. Department of Biochemistry, Medical Faculty, Dokuz Eylul University, 35340, Inciraltı-Izmir, Turkey

    Kemal Baysal

  3. TÜBİTAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, 21, 41470, Gebze-Kocaeli, Turkey

    Kemal Baysal & Zelal Adiguzel

  4. Department of Chemical Engineering, Boğaziçi University, 81815, Bebek-Istanbul, Turkey

    Bahattin M. Baysal

Authors
  1. Ayse Z. Aroguz
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  2. Kemal Baysal
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  3. Zelal Adiguzel
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  4. Bahattin M. Baysal
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Correspondence to Bahattin M. Baysal.

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Aroguz, A.Z., Baysal, K., Adiguzel, Z. et al. Alginate/Polyoxyethylene and Alginate/Gelatin Hydrogels: Preparation, Characterization, and Application in Tissue Engineering. Appl Biochem Biotechnol 173, 433–448 (2014). https://doi.org/10.1007/s12010-014-0851-0

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  • DOI: https://doi.org/10.1007/s12010-014-0851-0

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