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Purification and Characterization of Pepsin-Solubilized Collagen from Skin of Sea Cucumber Holothuria parva

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Abstract

Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum transition (Tm) and denaturation temperature (Td) of PSC were determined to be 46.94 and 32.5 °C, respectively. The amino acid composition analysis revealed that glycine, proline, alanine, and hydroxyproline were the abundant amino acids available in extracted PSC. The results showed that the isolated collagen from H. parva has some similar characteristics to previously reported collagens used in food and pharmaceutical industries.

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Acknowledgment

We would like to thank the National Institute of Genetic Engineering and Biotechnology (NIGEB) for providing the research facility.

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Correspondence to Saeed Aminzadeh.

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Adibzadeh, N., Aminzadeh, S., Jamili, S. et al. Purification and Characterization of Pepsin-Solubilized Collagen from Skin of Sea Cucumber Holothuria parva . Appl Biochem Biotechnol 173, 143–154 (2014). https://doi.org/10.1007/s12010-014-0823-4

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  • DOI: https://doi.org/10.1007/s12010-014-0823-4

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