Applied Biochemistry and Biotechnology

, Volume 170, Issue 2, pp 381–398

Isolation of Biphenyl and Polychlorinated Biphenyl-Degrading Bacteria and Their Degradation Pathway

Authors

    • Division of Applied Sciences, College of Environmental Technology, Graduate School of EngineeringMuroran Institute of Technology
  • Kazunori Takada
    • Division of Applied Sciences, College of Environmental Technology, Graduate School of EngineeringMuroran Institute of Technology
    • Biotechnology LabBK Company R&D Center
    • Department of Pharmacy, College of PharmacyChungbuk National University
  • Tadashi Toyama
    • Department of Research, Interdisciplinary Graduate School of Medical and EngineeringUniversity of Yamanashi
  • Ken Sawada
    • Division of Applied Sciences, College of Environmental Technology, Graduate School of EngineeringMuroran Institute of Technology
  • Shintaro Kikuchi
    • Division of Applied Sciences, College of Environmental Technology, Graduate School of EngineeringMuroran Institute of Technology
Article

DOI: 10.1007/s12010-013-0191-5

Cite this article as:
Chang, Y., Takada, K., Choi, D. et al. Appl Biochem Biotechnol (2013) 170: 381. doi:10.1007/s12010-013-0191-5

Abstract

Four strains of biphenyl-degrading bacteria were isolated from a sewage and identified from the Rhodococcus genus (SK-1, SK-3, and SK-4) and Aquamicrobium genus (SK-2) by 16S rRNA sequence. Among these strains, strain SK-2 was most suitable for biphenyl degradation. When 0.65, 1.3, 2.6, or 3.9 mM of biphenyl was used, the biphenyl was completely degraded within 24 and 96 h of culture, respectively. However, in the case of 6.5 and 9.75 mM of biphenyl, the biphenyl degradation yields were about 80 % and 46.7 % after 120 h of culture, respectively. The isolated strains could degrade a broad spectrum of aromatic compounds including high-chlorinated polychlorinated biphenyl (PCB) congeners in the presence of biphenyl. In addition, strain SK-2 could utilize PCB congeners containing one to six chlorine substituents such as 2,2′,4,4′,5,5′-hexachlorobiphenyl. The PCB utilization rate by the strain SK-2 was increased compared to that of other PCB congener-utilizing bacteria. The four isolates metabolized 4-chlorobiphenyl to 4-chlorobenzoic acid and 2-hydroxy-6-oxo-6-(4′-chlorophenyl)-hexa-2,4-dienoic acid. These results suggest the isolated strains might be good candidates for the bioremediation of PCB-contaminated soil, especially high-saline soils.

Keywords

Biphenyl Polychlorinated biphenyl (PCB) Biphenyl-degrading mechanism Rhodococcus genus Aquamicrobium genus

Copyright information

© Springer Science+Business Media New York 2013