Isolation of Biphenyl and Polychlorinated Biphenyl-Degrading Bacteria and Their Degradation Pathway
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Four strains of biphenyl-degrading bacteria were isolated from a sewage and identified from the Rhodococcus genus (SK-1, SK-3, and SK-4) and Aquamicrobium genus (SK-2) by 16S rRNA sequence. Among these strains, strain SK-2 was most suitable for biphenyl degradation. When 0.65, 1.3, 2.6, or 3.9 mM of biphenyl was used, the biphenyl was completely degraded within 24 and 96 h of culture, respectively. However, in the case of 6.5 and 9.75 mM of biphenyl, the biphenyl degradation yields were about 80 % and 46.7 % after 120 h of culture, respectively. The isolated strains could degrade a broad spectrum of aromatic compounds including high-chlorinated polychlorinated biphenyl (PCB) congeners in the presence of biphenyl. In addition, strain SK-2 could utilize PCB congeners containing one to six chlorine substituents such as 2,2′,4,4′,5,5′-hexachlorobiphenyl. The PCB utilization rate by the strain SK-2 was increased compared to that of other PCB congener-utilizing bacteria. The four isolates metabolized 4-chlorobiphenyl to 4-chlorobenzoic acid and 2-hydroxy-6-oxo-6-(4′-chlorophenyl)-hexa-2,4-dienoic acid. These results suggest the isolated strains might be good candidates for the bioremediation of PCB-contaminated soil, especially high-saline soils.
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- Isolation of Biphenyl and Polychlorinated Biphenyl-Degrading Bacteria and Their Degradation Pathway
Applied Biochemistry and Biotechnology
Volume 170, Issue 2 , pp 381-398
- Cover Date
- Print ISSN
- Online ISSN
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- Polychlorinated biphenyl (PCB)
- Biphenyl-degrading mechanism
- Rhodococcus genus
- Aquamicrobium genus
- Industry Sectors
- Author Affiliations
- 1. Division of Applied Sciences, College of Environmental Technology, Graduate School of Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran, 050-8585, Hokkaido, Japan
- 2. Biotechnology Lab, BK Company R&D Center, Jeonbuk, 579-879, Republic of Korea
- 3. Department of Pharmacy, College of Pharmacy, Chungbuk National University, Cheongju, 361-763, Republic of Korea
- 4. Department of Research, Interdisciplinary Graduate School of Medical and Engineering, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8511, Japan