Abstract
Thirty heterokaryons, formed by protoplast fusion of Aspergillus nidulans and Aspergillus tubingensis, were selected on the basis of their ability to grow on 2-deoxyglucose (0.2 %, w/v) and intermediate spore color. These heterokaryons were studied for cellulase production using shake flask and solid substrate cultures at 40 °C. Fusants 51 and 28 exhibited appreciably higher levels of endoglucanase, cellobiohydrolase, β-glucosidase, and FPase activities when compared with parental strains. Employing proteomic-based approaches, the differential expression of proteins in secretome of fusants and parental strains were analyzed using two-dimensional electrophoresis. The expression of some of the proteins in the fusants was found to be up/downregulated. The upregulated proteins in the fusant 51 were identified by liquid chromatography–mass spectroscopy as endoxylanase, endochitinase, β-glucosidase, as well as hypothetical proteins. The cellulases produced by fusants 28 and 51 showed improved saccharification of alkali treated rice straw when compared with the parental strains.
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Acknowledgment
The financial support from NAIP (ICAR) for carrying out this research project (NAIP/Comp-4/C-30030) “Novel biotechnological processes for production of high-value products from rice straw and bagasse” is duly acknowledged.
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Kaur, B., Sharma, M., Soni, R. et al. Proteome-Based Profiling of Hypercellulase-Producing Strains Developed Through Interspecific Protoplast Fusion Between Aspergillus nidulans and Aspergillus tubingensis . Appl Biochem Biotechnol 169, 393–407 (2013). https://doi.org/10.1007/s12010-012-9985-0
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DOI: https://doi.org/10.1007/s12010-012-9985-0