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Isolation of AtNUDT5 Gene Promoter and Characterization of Its Activity in Transgenic Arabidopsis thaliana

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Abstract

AtNUDT5 is a cytosol Nudix that catalyzes the hydrolysis of a variety of substrates. In this report, a 1,387-bp 5′-flanking region of the AtNUDT5 gene was isolated from Arabidopsis thaliana. The tissue-specific activity of the 5′-flanking region was investigated by using the GUS gene as a reporter in transgenic A. thaliana plants. Weak GUS activity appeared in vascular tissues of young plants, strong GUS activity appeared in the axial roots, but no GUS activity was observed in the root cap, lateral roots, rosette leaf, mature silique and reproductive tissues such as stamen, pistil, and petal. Furthermore, by using these transgenic A. thaliana plants, results of the histochemical staining and fluorometric assays of GUS activity showed that the AtNUDT5 promoter can be activated by both avirulent Pst avrRpm1 and virulent Pst strains at 5 h post-infiltration and that the activity of AtNUDT5 promoter increased significantly at 24 h post-infiltration. Taken together, our results demonstrated that the AtNUDT5 promoter is pathogen-responsive. The promoter may be used to develop transgenic plants with an increased tolerance to pathogenic stresses.

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Acknowledgments

This work was supported by Central Public-interest Scientific Institution Basal Research Fund (1630052012007) and Natural Science Foundation of China (30760196 and 31070608).

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, XueYuan Rd 4, Longhua District, Haikou, Hainan, People’s Republic of China, 571101

    Xiu-Chun Zhang, Mei-Ying Li, Meng-Bin Ruan, Yi-Ji Xia, Kun-Xin Wu & Ming Peng

  2. Environmental Safety Supervision and Inspection Centre for Genetically Modified Plants and Microorganisms Used in Plants, Ministry of Agriculture, XueYuan Rd 4, Longhua District, Haikou, Hainan, People’s Republic of China, 571101

    Xiu-Chun Zhang, Mei-Ying Li & Ming Peng

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  1. Xiu-Chun Zhang
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  2. Mei-Ying Li
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  3. Meng-Bin Ruan
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  4. Yi-Ji Xia
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Correspondence to Kun-Xin Wu or Ming Peng.

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Xiu-Chun Zhang and Mei-Ying Li contributed equally to the article.

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Zhang, XC., Li, MY., Ruan, MB. et al. Isolation of AtNUDT5 Gene Promoter and Characterization of Its Activity in Transgenic Arabidopsis thaliana . Appl Biochem Biotechnol 169, 1557–1565 (2013). https://doi.org/10.1007/s12010-012-0071-4

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  • DOI: https://doi.org/10.1007/s12010-012-0071-4

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