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The Utilization of Gum Tragacanth to Improve the Growth of Rhodotorula aurantiaca and the Production of γ-Decalactone in Large Scale

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Abstract

The production of γ-decalactone and 4-hydroxydecanoic acid by the psychrophilic yeast R. aurantiaca was studied. The effect of both compounds on the growth of R. aurantiaca was also investigated and our results show that γ-decalactone must be one of the limiting factors for its production. The addition of gum tragacanth to the medium at concentrations of 3 and 4 g/l seems to be an adequate strategy to enhance γ-decalactone production and to reduce its toxicity towards the cell. The production of γ-decalactone and 4-hydroxydecanoic acid was significantly higher in 20-l bioreactor than in 100-l bioreactor. By using 20 g/l of castor oil, 6.5 and 4.5 g/l of γ-decalactone were extracted after acidification at pH 2.0 and distillation at 100 °C for 45 min in 20- and 100-l bioreactors, respectively. We propose a process at industrial scale using a psychrophilic yeast to produce naturally γ-decalactone from castor oil which acts also as a detoxifying agent; moreover the process was improved by adding a natural gum.

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Acknowledgement

We thank the government of Syria for their financial support to carry out this study.

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Authors and Affiliations

  1. Faculté Universitaire des Sciences Agronomiques de Gembloux, Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Passage des Déportés, 2, 5030, Gembloux, Belgium

    Mohamed Alchihab, Jacqueline Destain & Philippe Thonart

  2. Faculté Universitaire des Sciences Agronomiques de Gembloux, Unité de Chimie Générale et Organique, Passage des Déportés, 2, 5030, Gembloux, Belgium

    Mario Aguedo & Jean-Paul Wathelet

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  1. Mohamed Alchihab
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  2. Jacqueline Destain
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  3. Mario Aguedo
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  4. Jean-Paul Wathelet
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  5. Philippe Thonart
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Correspondence to Mohamed Alchihab.

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Alchihab, M., Destain, J., Aguedo, M. et al. The Utilization of Gum Tragacanth to Improve the Growth of Rhodotorula aurantiaca and the Production of γ-Decalactone in Large Scale. Appl Biochem Biotechnol 162, 233–241 (2010). https://doi.org/10.1007/s12010-009-8739-0

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  • DOI: https://doi.org/10.1007/s12010-009-8739-0

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