Applied Biochemistry and Biotechnology

, Volume 160, Issue 2, pp 360–369

Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass

Authors

  • Yifeng Lu
    • State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology
    • State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology
  • Guoqian Xu
    • State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology
  • Ju Chu
    • State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology
  • Yingping Zhuang
    • State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology
  • Siliang Zhang
    • State Key Laboratory of Bioreactor EngineeringEast China University of Science and Technology
Article

DOI: 10.1007/s12010-008-8306-0

Cite this article as:
Lu, Y., Wang, Y., Xu, G. et al. Appl Biochem Biotechnol (2010) 160: 360. doi:10.1007/s12010-008-8306-0

Abstract

Steam-exploded corn stover biomass was used as the substrate for fed-batch separate enzymatic hydrolysis and fermentation (SHF) to investigate the solid concentration ranging from 10% to 30% (w/w) on the lignocellulose enzymatic hydrolysis and fermentation. The treatment of washing the steam-exploded material was also evaluated by experiments. The results showed that cellulose conversion changed little with increasing solid concentration, and fermentation by Saccharomyces cerevisiae revealed a nearly same ethanol yield with the water-washed steam-exploded corn stover. For the washed material at 30% substrate concentration, i.e., 30% water insoluble solids (WIS), enzymatic hydrolysis yielded 103.3 g/l glucose solution and a cellulose conversion of 72.5%, thus a high ethanol level up to 49.5 g/l. With the unwashed steam-exploded corn stover, though a cellulose conversion of 70.9% was obtained in hydrolysis at 30% solid concentration (27.9% WIS), its hydrolysate did not ferment at all, and the hydrolysate of 20% solid loading containing 3.3 g/l acetic acid and 145 mg/l furfural already exerted a strong inhibition on the fermentation and ethanol production.

Keywords

BioethanolCorn stoverHigh solid concentrationEnzymatic hydrolysisFermentation

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© Humana Press 2008