Applied Biochemistry and Biotechnology

, Volume 137, Issue 1, pp 909–925

Functional stability of a mixed microbial consortium producing PHA from waste carbon sources

  • Erik R. Coats
  • Frank J. Loge
  • William A. Smith
  • David N. Thompson
  • Michael P. Wolcott
Session 5: Bioprocessing And Separations R&D

DOI: 10.1007/s12010-007-9107-6

Cite this article as:
Coats, E.R., Loge, F.J., Smith, W.A. et al. Appl Biochem Biotechnol (2007) 137: 909. doi:10.1007/s12010-007-9107-6

Abstract

Polyhydroxyalkanoates (PHAs) represent an environmentally effective alternative to synthetic thermoplastics; however, current production practices are not sustainable. In this study, PHA production was accomplished in sequencing batch bioreactors utilizing real wastewaters and mixed microbial consortia from municipal activated sludge as inoculum. Polymer production reached 85, 53, and 10% of the cell dry weight from methanol-enriched pulp and paper mill foul condensate, fermented municipal primary solids, and biodiesel wastewater, respectively. Using denaturing gradient gel electrophoresis of 16S-rDNA from polymerase chain reaction-amplified DNA extracts, distinctly different communities were observed between and within wastewaters following enrichment. Most importantly, functional stability was maintained despite differing and contrasting microbial populations.

Index Entries

Activated sludgedenaturing gradient gel electrophoresispolyhydroxyalkanoateswastewaterprimary solids fermentatefoul condensateenvironmental biotechnology

Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Erik R. Coats
    • 1
  • Frank J. Loge
    • 2
  • William A. Smith
    • 3
  • David N. Thompson
    • 3
  • Michael P. Wolcott
    • 4
  1. 1.Department of Civil EngineeringUniversity of IdahoMoscow, Idaho
  2. 2.Department of Civil and Environmental EngineeringUniversity of California DavisDavis
  3. 3.Biotechnology DepartmentIdaho National LaboratoryIdaho Falls
  4. 4.Department of Civil and Environmental EngineeringWashington State UniversityPullman