Applied Biochemistry and Biotechnology

, Volume 143, Issue 2, pp 153–163

Enzyme–Carbon Nanotube Conjugates in Room-temperature Ionic Liquids

  • Bilge Eker
  • Prashanth Asuri
  • Saravanababu Murugesan
  • Robert J. Linhardt
  • Jonathan S. Dordick
Article

DOI: 10.1007/s12010-007-0035-2

Cite this article as:
Eker, B., Asuri, P., Murugesan, S. et al. Appl Biochem Biotechnol (2007) 143: 153. doi:10.1007/s12010-007-0035-2

Abstract

Room-temperature ionic liquids (RTILs) are intriguing solvents, which are recognized as “green” alternatives to volatile organics. Although RTILs are nonvolatile and can dissolve a wide range of charged, polar, and nonpolar organic and inorganic molecules, there remain substantial challenges in their use, not the least of which is the solvents’ high viscosity that leads to potential mass transfer limitations. In the course of this work, we discovered that the simple adsorption of the bacterial protease, proteinase K, onto single-walled carbon nanotubes (SWNTs) results in intrinsically high catalytic turnover. The high surface area and the nanoscopic dimensions of SWNTs offered high enzyme loading and low mass transfer resistance. Furthermore, the enzyme–SWNT conjugates displayed enhanced thermal stability in RTILs over the native suspended enzyme counterpart and allowed facile reuse. These enzyme–SWNT conjugates may therefore provide a way to overcome key operational limitations of RTIL systems.

Keywords

Room-temperature ionic liquids Enzyme–SWNT conjugates Diffusional limitations Enzyme kinetics Thermostability Reusability 

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Bilge Eker
    • 1
  • Prashanth Asuri
    • 1
  • Saravanababu Murugesan
    • 1
  • Robert J. Linhardt
    • 1
    • 2
    • 3
  • Jonathan S. Dordick
    • 1
    • 2
  1. 1.Department of Chemical and Biological Engineering, Rensselaer Nanotechnology Center, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  2. 2.Department of Biology, Rensselaer Nanotechnology Center, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  3. 3.Department of Chemistry, Rensselaer Nanotechnology Center, Center for Biotechnology and Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA