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Use of Electrospinning to Develop Antimicrobial Biodegradable Multilayer Systems: Encapsulation of Cinnamaldehyde and Their Physicochemical Characterization

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Abstract

In this work, three active bio-based multilayer structures, using a polyhydroxybutyrate-co-valerate film with a valerate content of 8 % (PHBV8) as support, were developed. To this end, a zein interlayer with or without cinnamaldehyde (CNMA) was directly electrospun onto one side of the PHBV8 film and the following systems were developed: (1) without an outer layer; (2) using a PHBV8 film as outer layer; and (3) using an alginate-based film as outer layer. These multilayer structures were characterized in terms of water vapour and oxygen permeabilities, transparency, intermolecular arrangement and thermal properties. The antimicrobial activity of the active bio-based multilayer systems and the release of CNMA in a food simulant were also evaluated. Results showed that the presence of different outer layers reduced the transport properties and transparency of the multilayer films. The active bio-based multilayer systems showed antibacterial activity against Listeria monocytogenes being the multilayer structure prepared with CNMA and PHBV outer layers (PHBV + zein/CNMA + PHBV) the one that showed the greater antibacterial activity. The release of CNMA depended on the multilayer structures, where both Fick’s and Case II transport—polymer relaxation explained the release of CNMA from the multilayer systems.

Overall, the deposition of electrospun CNMA-loaded zein fibres on a PHBV8 layer is a promising methodology for the development of active bio-based multilayer systems, with a great potential for food packaging applications.

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Acknowledgments

Miguel A. Cerqueira (SFRH/BPD/72753/2010) and Ana I. Bourbon (SFRH/BD/73178/2010) are recipient of a fellowship from the Fundação para a Ciência e Tecnologia (FCT, POPH-QREN and FSE Portugal). J.L. Castro-Mayorga is supported by the Administrative Department of Science, Technology and Innovation (Colciencias) of Colombian Government. M. J. Fabra is a recipient of a Ramon y Cajal contract (RyC-2014-158) from the Spanish Ministry of Economy and Competitiveness. This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). The support of EU Cost Action MP1206 is gratefully acknowledged.

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Authors and Affiliations

  1. Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Miguel A. Cerqueira, Ana I. Bourbon & António A. Vicente

  2. INL—International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, 4715-330, Braga, Portugal

    Miguel A. Cerqueira & Lorenzo M. Pastrana

  3. Novel Materials and Nanotechnology Group, IATA-CSIC, Av. Agustin Escardino 7, 46980, Paterna (Valencia), Spain

    María José Fabra, Jinneth Lorena Castro-Mayorga & Jose M. Lagaron

Authors
  1. Miguel A. Cerqueira
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  2. María José Fabra
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  3. Jinneth Lorena Castro-Mayorga
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  4. Ana I. Bourbon
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  5. Lorenzo M. Pastrana
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  6. António A. Vicente
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  7. Jose M. Lagaron
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Correspondence to Miguel A. Cerqueira or María José Fabra.

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Cerqueira, M.A., Fabra, M.J., Castro-Mayorga, J.L. et al. Use of Electrospinning to Develop Antimicrobial Biodegradable Multilayer Systems: Encapsulation of Cinnamaldehyde and Their Physicochemical Characterization. Food Bioprocess Technol 9, 1874–1884 (2016). https://doi.org/10.1007/s11947-016-1772-4

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  • DOI: https://doi.org/10.1007/s11947-016-1772-4

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