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Innovative Graphite Oxide-Cellulose Based Material Specific for Genomic DNA Extraction

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Abstract

Extraction of genomic DNA from various types of samples is often challenging for commercial silica spin column. In this study, we proposed graphite oxide (GO)/cellulose composite as an alternative material for genomic DNA extraction. The purity of DNA and extraction efficiency were compared to that of commercial silica product. In this study, the total weight % of GO was fixed at 4.15% in GO/Cellulose composite. Chewed gum, nail clip, cigarette bud paper, animal tissue and hair sample were used as various genomic DNA sources for extraction experiments. Among all types of samples, the extraction efficiencies were 4 to 12 times higher than that of commercial silica spin column. The absorbance ratio of 260 nm to 280 nm (A260/A280) of all samples ranged between 1.6 and 2.0. The results demonstrated that GO/Cellulose composites might serve as an innovative solid support material for genomic DNA extraction.

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Authors and Affiliations

  1. Department of Materials, Physics and Energy Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, 464-8603, Japan

    Garbis Atam Akceoglu, Oi Lun Li & Nagahiro Saito

  2. Green Mobility Collaborative Research Center, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, 464-8603, Japan

    Oi Lun Li & Nagahiro Saito

  3. Socıal Innovation Design Center (SIDC), Instıtute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya, 464-8603, Japan

    Nagahiro Saito

Authors
  1. Garbis Atam Akceoglu
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  2. Oi Lun Li
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  3. Nagahiro Saito
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Correspondence to Nagahiro Saito.

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Akceoglu, G.A., Li, O.L. & Saito, N. Innovative Graphite Oxide-Cellulose Based Material Specific for Genomic DNA Extraction. JOM 67, 2557–2563 (2015). https://doi.org/10.1007/s11837-015-1610-6

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  • DOI: https://doi.org/10.1007/s11837-015-1610-6

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