Arthropod-Plant Interactions

, Volume 10, Issue 2, pp 143–150

Chew and spit: tree-feeding notodontid caterpillars anoint girdles with saliva

  • David E. Dussourd
  • Michelle Peiffer
  • Gary W. Felton
Original Paper

DOI: 10.1007/s11829-016-9416-1

Cite this article as:
Dussourd, D.E., Peiffer, M. & Felton, G.W. Arthropod-Plant Interactions (2016) 10: 143. doi:10.1007/s11829-016-9416-1

Abstract

Caterpillars of the notodontid Oedemasia leptinoides (formerly Schizura) use their mandibles to cut shallow girdles that encircle the petioles and stems of tree hosts. When girdles are complete, the larvae bathe the girdle surface with fluid. We test whether the fluid originates from the labial salivary glands or ventral eversible gland by blocking the openings to the glands and observing whether fluid is still released onto the girdles. Only larvae with functional labial salivary glands anointed girdles with fluid. Analysis of girdle rinses for a prominent salivary enzyme, glucose oxidase, confirmed that larvae apply saliva and documented that application occurs primarily at the end of girdling. We propose that girdling by notodontids, together with related furrowing and leaf-clipping behaviors exhibited by diverse caterpillar groups, serve at least in part to introduce salivary components to exposed vascular tissues; these compounds presumably function to suppress plant defensive responses normally elicited by caterpillar feeding.

Keywords

Girdling behavior Saliva Glucose oxidase Labial salivary gland Oedemasia leptinoides Notodontidae 

Supplementary material

11829_2016_9416_MOESM1_ESM.m2v (149.3 mb)
Movie 1 Final instar Oedemasia leptinoides larva cutting a girdle in a pecan rachis. After 24 s, the film speed is gradually increased to ten times normal speed (m2v 152834 kb)
View video

Movie 2 Closeup of a final instar Oedemasia leptinoides larva completing a girdle in a pecan rachis. The white fibrous girdle appears dry during the cutting procedure. At the end, the larva moves its head side to side as it rubs its spinneret back and forth over the surface, painting the girdle with saliva (mpg 121514 kb)

11829_2016_9416_MOESM3_ESM.m2v (20.5 mb)
Movie 3 Final instar Oedemasia leptinoides on filter paper impregnated with alkaline phenolphthalein. Contact with forceps caused the larva to discharge its VEG repeatedly, each time expelling formic acid directly at the forceps causing the pH sensitive paper to change from pink to white (m2v 21020 kb)
11829_2016_9416_MOESM4_ESM.m2v (232.9 mb)
Movie 4 Final instar Schizura ipomoeae completing a girdle in river birch, Betula nigra. During and especially after girdling, the larva wiped its labium over the girdle surface, thereby drenching the girdle in fluid, presumably saliva, as documented with O. leptinoides (m2v 238532 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • David E. Dussourd
    • 1
  • Michelle Peiffer
    • 2
  • Gary W. Felton
    • 2
  1. 1.Department of BiologyUniversity of Central ArkansasConwayUSA
  2. 2.Department of EntomologyPenn State UniversityUniversity ParkUSA