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A simultaneous microwave-assisted extraction and adsorbent treatment process under acidic conditions for recovery and separation of paclitaxel from plant cell cultures

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Abstract

We have developed a simultaneous microwave-assisted extraction and adsorbent treatment process under acidic conditions to increase the recovery and separation efficiency of the anticancer agent paclitaxel from plant cell culture. The simultaneous process under the conditions of extracting solution (90% aqueous methanol), pH 2.2, extraction time, 6min, ratio of extracting solution to biomass, 1: 1 (v/w), extraction temperature, 40 °C, adsorbent type, sylopute, and ratio of adsorbent to biomass, 0.08: 1 (w/w), facilitated 1.97-fold higher recovery of paclitaxel in a shorter extraction time than the conventional solvent extraction process. In addition, biomass-derived tar compounds were successfully removed by the simultaneous process alone (average removal >97%). Using the simultaneous process, the paclitaxel extraction efficiency was improved, biomass-derived tar compounds were removed, and the purification process was simplified at the same time.

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References

  1. M.C. Wani, H. L. Taylor, M. E. Wall, P. Coggon and A.T. McPhail, J. Am. Chem. Soc., 93, 2325 (1971).

    Article  CAS  Google Scholar 

  2. J. H. Kim, Korean J. Biotechnol. Bioeng., 21, 1 (2006).

    Google Scholar 

  3. W. P. McGuire, E. K. Rowinsky, N.B. Rosenhein, F.C. Grumbine, D. S. Ettinger, D.K. Armstrong and R.C. Donehower, Int. J. Gynecol. Obstet., 31, 298 (1990).

    Article  Google Scholar 

  4. J.R. Hsiao, S.F. Leu and B.M. Huang, J. Oral Pathol. Med., 38, 188 (2009).

    Article  CAS  Google Scholar 

  5. K. Rao, J. Hanuman, C. Alvarez, M. Stoy, J. Juchum, R. Davies and R. Baxley, Pharm. Res., 12, 1003 (1995).

    Article  CAS  Google Scholar 

  6. H. K. Choi, J. S. Son, G. H. Na, S. S. Hong, Y. S. Park and J.Y. Song, Korean J. Plant Biotechnol., 29, 59 (2002).

    Article  Google Scholar 

  7. E. Baloglu and D. G. I. Kingston, J. Nat. Prod., 62, 1068 (1999).

    Article  CAS  Google Scholar 

  8. B. Zhang, R. Yang and C.Z. Liu, Sep. Purif. Technol., 62, 480 (2008).

    Article  CAS  Google Scholar 

  9. W. K. Kim, H. J. Chae and J. H. Kim, Biotechnol. Bioproc. Eng., 15, 481 (2010).

    Article  CAS  Google Scholar 

  10. J. H Kwon, Y. H. Choi, H.W. Chung and G.D. Lee, Intern. J. Food Sci. Technol., 41, 67 (2006).

    Article  CAS  Google Scholar 

  11. D. P. Fulzele and R.K. Satdive, J. Chromatogr. A, 1063, 9 (2005).

    Article  CAS  Google Scholar 

  12. F. Chen, K. Mo, Z. Liu, F. Yang, K. Hou, S. Li, Y. Zu and L. Yang, Molecules, 19, 9689 (2014).

    Article  CAS  Google Scholar 

  13. Y. Y. Shu, M. Y. Ko and Y. S. Chang, Microchem. J., 74, 131 (2003).

    Article  CAS  Google Scholar 

  14. X. Pan, H. Liu, G. Jia and Y.Y. Shu, Biochem. Eng. J., 5, 173 (2000).

    Article  CAS  Google Scholar 

  15. X. Pan, G. Niu and H. Liu, Chem. Eng. Process., 42, 129 (2003).

    Article  CAS  Google Scholar 

  16. J. E. Hyun and J. H. Kim, Korean J. Biotechnol. Bioeng., 23, 281 (2008).

    Google Scholar 

  17. J. Y. Lee and J. H. Kim, Sep. Purif. Technol., 80, 240 (2011).

    Article  CAS  Google Scholar 

  18. G. J. Kim and J. H. Kim, Process Biochem., In Press(2014).

    Google Scholar 

  19. H. K. Choi, T. L. Adams, R.W. Stahlhut, S. I. Kim, J. H. Yun, B. K. Song, J. H. Kim, J. S. Song, S. S. Hong and H. S. Lee, US Patent, 5,871,979 (1999).

    Google Scholar 

  20. K. Y. Jeon and J. H. Kim, Korean J. Biotechnol. Bioeng., 23, 557 (2008).

    Google Scholar 

  21. G. J. Kim, G.Y. Park and J. H. Kim, Korean J. Microbiol. Biotechnol., 41, 272 (2013).

    Article  CAS  Google Scholar 

  22. Y. L. Jeon and J.H. Kim, Korean J. Chem. Eng., 30, 1954 (2013).

    Article  CAS  Google Scholar 

  23. H. J. Oh, H.R. Jang, K.Y. Jung and J. H. Kim, Process Biochem., 47, 331 (2012).

    Article  CAS  Google Scholar 

  24. G. Y. Park, G. J. Kim and J. H. Kim, J. Ind. Eng. Chem., 21, 151 (2015).

    Article  CAS  Google Scholar 

  25. Y. Chen, M. Y. Xie and X. F. Gong, J. Food Eng., 81, 162 (2007).

    Article  CAS  Google Scholar 

  26. M. Ramil Criado, S. Pombo Da Torre, I. RodríGuez Pereiro and R. Cela Torrijos, J. Chromatogr. A, 1024, 155 (2004).

    Article  Google Scholar 

  27. M. Gfrerer and E. Lankmayr, Anal. Chim. Acta, 533, 203 (2005).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Kongju National University, Cheonan, 330-717, Korea

    Gun-Joong Kim & Jin-Hyun Kim

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  1. Gun-Joong Kim
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  2. Jin-Hyun Kim
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Correspondence to Jin-Hyun Kim.

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Kim, GJ., Kim, JH. A simultaneous microwave-assisted extraction and adsorbent treatment process under acidic conditions for recovery and separation of paclitaxel from plant cell cultures. Korean J. Chem. Eng. 32, 1023–1028 (2015). https://doi.org/10.1007/s11814-015-0075-1

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  • DOI: https://doi.org/10.1007/s11814-015-0075-1

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