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Effects of Particle Size of Sucrose Suspensions and Pre-incubation of Enzymes on Lipase-Catalyzed Synthesis of Sucrose Oleic Acid Esters

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Journal of the American Oil Chemists' Society

Abstract

The effects of high-speed homogenization, high-intensity ultrasound, and their combination were evaluated for the reduction of the particle size of sucrose crystals to enhance solvent-free lipase-catalyzed synthesis of sucrose oleate at 65 °C. The combination of homogenization and ultrasound greatly decreased the particle size of suspended sucrose crystals in mixtures of oleic acid/sucrose oleate (86 wt% monoester and 14 wt% diester at a ratio of 90/10 w/w) from 88 to 18 μm. The suspension-based medium was charged to a stirred tank bioreactor that also contained immobilized lipase from Rhizomucor miehei or Candida antarctica (Lipozyme®IM and Novozym® 435, respectively; Novozymes, Franklinton, NC, USA), that was pre-incubated in oleic acid for several different temperatures (23–60 °C), durations (4–24 h), and stir rates (50–400 rpm, radius of 3 cm), prior to use. The optimal performance was achieved using C. antarctica lipase (83.3 wt% ester, consisting of 46 wt% monoester) in the presence of molecular sieves (18 wt%). The low water concentration (~0.12 wt%) did not affect the activity of C. antarctica lipase.

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Acknowledgments

Financial support was kindly supplied by the US Department of Agriculture, SBIR Grant (2012-33610-19502). We also thank Dr. Svetlana Zivanovic for her assistance in the use of high speed homogenizator and ultrasound instruments.

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Authors and Affiliations

  1. Department of Biosystems Engineering and Soil Science, University of Tennessee, 2506 E.J. Chapman Dr., Knoxville, TN, 37996-4531, USA

    Ran Ye & Douglas G. Hayes

  2. MARC-IV Consulting Inc., 101 E. Main Street, Kearney, MO, 64060, USA

    Rachel Burton

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  1. Ran Ye
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  2. Douglas G. Hayes
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Correspondence to Douglas G. Hayes.

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Ye, R., Hayes, D.G. & Burton, R. Effects of Particle Size of Sucrose Suspensions and Pre-incubation of Enzymes on Lipase-Catalyzed Synthesis of Sucrose Oleic Acid Esters. J Am Oil Chem Soc 91, 1891–1901 (2014). https://doi.org/10.1007/s11746-014-2537-8

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  • DOI: https://doi.org/10.1007/s11746-014-2537-8

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