Journal of the American Oil Chemists' Society

, Volume 89, Issue 2, pp 189–198

Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae

  • Eline Ryckebosch
  • Koenraad Muylaert
  • Imogen Foubert
Original Paper

DOI: 10.1007/s11746-011-1903-z

Cite this article as:
Ryckebosch, E., Muylaert, K. & Foubert, I. J Am Oil Chem Soc (2012) 89: 189. doi:10.1007/s11746-011-1903-z
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Abstract

An optimized procedure for extraction of total and non-polar lipids from microalgae is proposed. The effects of solvent, pretreatment (lyophilization, inactivation of lipases, and addition of antioxidants) and cell-disruption (liquid nitrogen, sonication, and bead beating) on total lipid content, lipid class, and fatty acid composition were examined. Chloroform–methanol 1:1 was shown to be the best solvent mixture for extraction of total lipids from microalgae. When performing this extraction, lyophilized algae can be used, no pretreatment with isopropanol to inactivate the lipases is needed and addition of antioxidants is not necessary. Furthermore, cell-disruption is not essential, although in that case two extractions must be performed in series to ensure that, irrespective of the microalgal species, all lipids are extracted. Determination of non-polar lipid content should be performed by separation of the total lipid extract on an SPE column. Extraction using petroleum ether is only appropriate when a bead beater is used for pretreatment.

Keywords

AlgaeLipid extractionSolvent mixturePretreatmentCell-disruptionFat content

Copyright information

© AOCS 2011

Authors and Affiliations

  • Eline Ryckebosch
    • 1
  • Koenraad Muylaert
    • 2
  • Imogen Foubert
    • 1
  1. 1.K. U. Leuven Kulak, Research Unit Food and Lipids, Department of Molecular and Microbial SystemsLeuven Food and Nutrition Research Centre (LFoRCe)KortrijkBelgium
  2. 2.K. U. Leuven Kulak, Lab Aquatic BiologyBiology DepartmentKortrijkBelgium