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Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed (Cannabis sativa L.) Protein Hydrolysates

  • Original Article
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Journal of the American Oil Chemists' Society

Abstract

The aim of this study was to determine the antihypertensive effects of enzymatic hemp seed protein hydrolysate (HPH) and its peptide fractions. Hemp seed protein isolate was digested by the sequential action of pepsin and pancreatin to mimic gastrointestinal digestion in human beings. The resultant HPH was separated by membrane ultrafiltration into peptide fractions with different sizes (<1 and 1–3 kDa). The HPH led to significantly higher (P < 0.05) in vitro inhibition of the activities of angiotensin I-converting enzyme (ACE) and renin, the two main enzymes involved in abnormal blood pressure elevation (hypertension). Kinetic studies showed that HPH and peptide fractions inhibited renin and ACE activities in a mixed-type pattern, indicating binding to areas other than the active site. Oral administration of HPH (200 mg/kg body weight) to spontaneously hypertensive rats led to significant reductions (P < 0.05) in systolic blood pressure (SBP) that reached a maximum of −30 mmHg after 8 h. In contrast, the hypotensive effects of peptide fractions (<1 and 1–3 kDa) had a maximum value of about −15 mmHg after 6–8 h post oral administration. The results suggest a synergistic antihypertensive effect of the peptides present within HPH; this effect was reduced significantly (P < 0.05) upon separation into peptide fractions.

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References

  1. Hong F, Ming L, Yi S, Zhanxia L, Yongquan W, Chi L (2008) The antihypertensive effect of peptides: A novel alternative to drugs? Peptides 29:1062–1071

    Article  Google Scholar 

  2. Aluko RE (2008) Antihypertensive properties of plant-derived inhibitors of angiotensin I-converting enzyme activity: a review. Recent Prog Med Plants 22:541–561

    CAS  Google Scholar 

  3. Atkinson AB, Robertson JIS (1979) Captopril in the treatment of clinical hypertension and cardiac. Lancet 314:836–839

    Article  Google Scholar 

  4. Lahogue V, Rehel K, Taupin L, Haras D, Allaume P (2010) A HPLC-UV method for the determination of angiotensin I-converting enzyme (ACE) inhibitory activity. Food Chem 118:870–875

    Article  CAS  Google Scholar 

  5. Erdmann K, Cheung BLY, Schroder H (2008) The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular disease. J Nutr Biochem 19:643–654

    Article  CAS  Google Scholar 

  6. Wang XS, Tang CH, Chen L, Yang XQ (2009) Characterization and antioxidant properties of hemp protein hydrolysate obtained with neutrase. Food Technol Biotechnol 47:428–434

    CAS  Google Scholar 

  7. Tang CH, Ten Z, Wang XS, Yang XQ (2006) Physicochemial and functional properties of hemp (Cannabis sativa L.) protein isolate. J Agric Food Chem 54:8945–8950

    Article  CAS  Google Scholar 

  8. Wang XS, Tang CH, Yang XQ, Gao WR (2008) Characterization amino acid composition and in vitro digestibility of hemp (Cannabis sativa L.) proteins. Food Chem 107:11–18

    Article  CAS  Google Scholar 

  9. House JD, Neufeld J, Leson G (2010) Evaluating the quality of protein from hemp seed (Cannabis sativa L.) products through the use of the protein digestibility-corrected amino acid score method. J Agric Food Chem 58:11801–11807

    Article  CAS  Google Scholar 

  10. Pihlanto LA, Koskinen P, Piilola K, Tupasela T, Korhonen H (2000) Angiotensin I-converting enzyme inhibitory properties of whey protein digests: concentration and characterization of active peptides. J Diary Res 67:53–64

    Article  Google Scholar 

  11. Girgih, AT, Udenigwe CC, Aluko RE (2011) In vitro antioxidant properties of hemp seed (Cannabis sativa L.) protein hydrolysate fractions. J Am Oil Chem Soc 88:381–389

    Google Scholar 

  12. FitzGerald RJ, Murray BA, Walsh DJ (2004) Hypotensive peptides from milk proteins. J Nutr 134:9805–9885

    Google Scholar 

  13. Udenigwe CC, Lin YS, Hou WC, Aluko RE (2009) Kinetics of the inhibition of renin and angiotensin I-converting by flaxseed protein hydrolysate fractions. J Funct Foods 1:199–207

    Article  Google Scholar 

  14. Segall L, Covic A, Goldsmith DJA (2007) Direct renin inhibitors: the dawn of a new era, or just a variation on the theme? Nephrol. Dial Transplant 22:2435–2439

    Article  Google Scholar 

  15. Li H, Aluko RE (2010) Identification and inhibitory properties of multifunctional peptides from pea protein hydrolysate. J Agric Food Chem 58:11471–11476

    Article  CAS  Google Scholar 

  16. Markwell MAC, Haas SM, Biebar LL, Tolbert NE (1978) A modification of lowry procedure to simplify protein determination in membrane and protein samples. Anal Biochem 87:206–211

    Article  CAS  Google Scholar 

  17. Adler-Nissen J (1979) Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid. J Agric Food Chem 27:1256–1262

    Article  CAS  Google Scholar 

  18. Li H, Aluko RE (2005) Kinetics of the inhibition of calcium/calmodulin-dependent protein kinase II by pea protein-derived peptides. J Nutr Biochem 16:656–662

    Article  CAS  Google Scholar 

  19. Aukema HM, Gauthier J, Jia Y, Roy M, Li H, Aluko RE (2011). Distinctive effects of plant protein sources on renal disease progression and associated cardiac hypertrophy in experimental kidney disease. Mol Nutr Food Res. doi: 10.1002/mnfr.201000558

  20. Giménez B, Alemán A, Montero P, Gómez-Guillén MC (2009) Antioxidant and functional properties of gelatin hydrolysates obtained from skin of sole and squid. Food Chem 114:976–983

    Article  Google Scholar 

  21. Yin SW, Tang CH, Cao JS, Wen HuEK, QB YangXQ (2008) Effects of limited enzymatic hydrolysis with trypsin on functional properties of hemp (Cannabis sativa L.) protein isolate. Food Chem 106:1004–1013

    Article  CAS  Google Scholar 

  22. Barbana C, Boye JI (2010) Angiotensin I-converting enzyme inhibitory activity of chickpea and pea protein hydrolysates. Food Res Int 43:1642–1649

    Article  CAS  Google Scholar 

  23. Shahidi F, Han X-Q, Synowiecki J (1995) Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem 53:285–293

    Article  CAS  Google Scholar 

  24. Benjakul S, Morrissey MT (1997) Protein hydrolysates from pacific whiting solid wastes. J Agric Food Chem 45:3423–3430

    Article  CAS  Google Scholar 

  25. Roberts PR, Burney JD, Black KW, Zaloga GP (1999) Effect of chain length on absorption of biologically active peptides from the gastrointestinal tract. Digestion 60:332–337

    Article  CAS  Google Scholar 

  26. Haque E, Chand R (2008) Antihypertensive and antimicrobial bioactive peptides from milk proteins. Eur Food Res Technol 277:7–15

    Article  Google Scholar 

  27. Belem MAF, Gibbs BF, Lee BH (1999) Proposing sequences for peptides derived from whey fermentation with potential bioactive sites. J Diary Sci 82:486–493

    Article  CAS  Google Scholar 

  28. Jeon Y-J, Byun H-G, Kim S-K (1999) Improvement of functional properties of cod frame protein hydrolysates using ultrafiltration membranes. Process Biochem 35:471–478

    Article  Google Scholar 

  29. Wu J, Aluko RE, Nakai S (2006) Structural requirements of angiotensin I-converting enzyme inhibitory peptides: quantitative structure-and-activity relationship study of di- and tri-peptides. J Agric Food Chem 54:732–738

    Article  CAS  Google Scholar 

  30. Stanton A (2003) Potential of renin inhibition in cardiovascular disease. J Renin Angiotensin Aldosterone Syst 4:6–10

    Article  CAS  Google Scholar 

  31. Qian ZJ, Je JY, Kim SK (2007) Antihypertensive effect of angiotensin I-converting enzyme-inhibitory peptide from hydrolysates of bigeye tuna dark muscle, Thunnus obesus. J Agric Food Chem 55:8398–8403

    Article  CAS  Google Scholar 

  32. Lee S-H, Qian Z-J, Kim S-K (2010) A novel angiotensin I converting enzyme inhibitory peptide from tuna frame protein hydrolysate and its antihypertensive effect in spontaneously hypertensive rats. Food Chem 118:96–102

    Article  CAS  Google Scholar 

  33. Wang J, Hu J, Cui J, Bai X, Du Y, Miyaguchi Y, Lin B (2008) Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the antihypertensive effect of hydrolysate in spontaneously hypertensive rats. Food Chem 111:302–308

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was funded through a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to R.E.A.

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Authors and Affiliations

  1. Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Abraham T. Girgih, Chibuike C. Udenigwe, Huan Li, Abayomi P. Adebiyi & Rotimi E. Aluko

  2. The Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Abayomi P. Adebiyi

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  1. Abraham T. Girgih
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  2. Chibuike C. Udenigwe
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  3. Huan Li
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  4. Abayomi P. Adebiyi
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  5. Rotimi E. Aluko
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Correspondence to Rotimi E. Aluko.

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Girgih, A.T., Udenigwe, C.C., Li, H. et al. Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed (Cannabis sativa L.) Protein Hydrolysates. J Am Oil Chem Soc 88, 1767–1774 (2011). https://doi.org/10.1007/s11746-011-1841-9

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  • DOI: https://doi.org/10.1007/s11746-011-1841-9

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