Skip to main content
SpringerLink
Log in

Biosurfactant from Lysinibacillus chungkukjangi from Rice Bran Oil Sludge and Potential Applications

  • Original Article
  • Published:
Journal of Surfactants and Detergents

Abstract

A newly discovered bacterium, Lysinibacillus chungkukjangi, was isolated from the sludge of rice bran oil processing. When the bacterium was grown on rice bran, it produced biosurfactant which reduced the surface tension of the media to 27.9 from 72 mN/m. The biosurfactant was recovered by a solvent extraction method and characterized with the help of various structure elucidation techniques viz. FTIR, 1H- and 13C-NMR spectroscopy and LC–MS analysis. The combined results of FTIR and NMR revealed the presence of carbonyl, olefinic and aliphatic groups, with the typical spectra of lipids. Moreover, LC–MS analysis also supported the same information. The biosurfactant was also studied for its anti-oxidant and microbial enhanced oil recovery (MEOR) potential. The anti-oxidant activity was observed by the DPPH free radical scavenging method using ascorbic acid as the standard. The IC50 (the half maximal inhibitory concentration) was calculated and for standard, it was 0.056 mg/mL and for the biosurfactant it turned out to be 1.3 mg/mL which shows its good anti-oxidant potential. The sandpack test was performed to check its MEOR potential and kerosene was recovered up to 90 %, which shows its excellent applicability in the MEOR processes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Vater J, Kablitz B, Wilde C, Franke P, Mehta N, Cameotra SS (2002) Matrix-assisted laser desorption ionization-time of flight mass spectrometry of lipopeptide biosurfactants in whole cells and culture filtrates of Bacillus subtilis C-1 isolated from petroleum sludge. Appl Environ Microbiol 68:6210–6219

    Article  CAS  Google Scholar 

  2. Biria D, Maghsoudi E, Roostaazad R, Dadafarin H, Lotfi AS, Amoozegar MA (2010) Purification and characterization of a novel biosurfactant produced by Bacillus licheniformis MS3. World J Microbiol Biotechnol 26:871–878

    Article  CAS  Google Scholar 

  3. Fracchi L, Cavallo M, Martinotti MG, Banat IM (2012) Biosurfactants and bioemulsifiers biomedical and related applications-present status and future potentials. In: Dhanjoo N, Ghista (eds) Biomedical Science, Engineering and Technology. In Tech, pp 325–370

  4. Bhardwaj G, Cameotra SS, Chopra HK (2013) Biosurfactants from fungi: a review. J Petro Environ Biotechnol 4:160

    Google Scholar 

  5. Bhardwaj G, Cameotra SS, Chopra HK (2013) Utilization of oleo-chemical industry by-products for biosurfactant production. AMB Express 3:68

    Article  Google Scholar 

  6. Morikawa M, Daido H, Takao T, Murata S, Shimonoshi Y, Imanaka T (1993) A new lipopeptide biosurfactant produced by Arthrobacter sp. Strain MIS38. J Bacteriol 175:6459–6466

    CAS  Google Scholar 

  7. Yakimov MM, Timmis KN, Wray V, Fredrickson HL (1995) Characterization of a new lipopeptide surfactant produced by thermotolerant and halotolerant subsurface Bacillus licheniformis BAS50. Appl Environ Biotechnol 61:1706–1713

    CAS  Google Scholar 

  8. Kim HS, Yoon BD, Lee CH, Suh HH, Oh HM, Katsuragi T, Tani Y (1997) Production and properties of lipopeptide biosurfactant from Bacillus subtilis C9. J Fermen Bioeng 84:41–46

    Article  Google Scholar 

  9. Fox SL, Bala GA (2000) Production of surfactant from Bacillus subtilis ATCC 21332 using potato substrates. Bioresour Technol 75:235–240

    Article  CAS  Google Scholar 

  10. Anyanwu CU, Obi SKC, Okolo BN (2011) Lipopeptide biosurfactant production by Serratia marcescens NSK-1 strain isolated from petroleum contaminated soil. J Appl Sci Res 7:79–87

    CAS  Google Scholar 

  11. Mandal SM, Sharma S, Pinnaka AK, Kumari A, Korpole S (2013) Isolation and characterization of diverse antimicrobial lipopeptides produced by Citrobacter and Enterobacter. BMC Microbiol 13:152

    Article  CAS  Google Scholar 

  12. Katz E, Demain AL (1977) The peptide antibiotics of Bacillus: chemistry, biogenesis and possible functions. Bacteriol Rev 41:449–474

    CAS  Google Scholar 

  13. Baindara P, Mandal SM, Chawla N, Singh PK, Pinnaka AK (2013) Characterization of two antimicrobial peptides produced by a halotolerant Bacillus subtilis strain SK.DU.4 isolated from a rhizosphere soil sample. AMB Express 3:2

    Article  Google Scholar 

  14. Bhardwaj G, Cameotra SS, Chopra HK (2015) Utilization of oil industry residues for the production of rhamnolipids by Pseudomonas indica. J Surfact Deterg 18:887–893

    Article  CAS  Google Scholar 

  15. Yalçin E, Çavuşoglu K (2010) Structural analysis and antioxidant activity of a biosurfactant obtained from Bacillus subtilis RW-I. Turk J Biochem 35:243–247

    Google Scholar 

  16. Pruthi V, Cameotra SS (1997) Production and properties of a biosurfactant synthesized by Arthrobacter protophormiae- an antarctic strain. World J Microbiol Biotechnol 13:137–139

    Article  CAS  Google Scholar 

  17. Aparna A, Srinikethan G, Smitha H (2012) Production and characterization of biosurfactant produced by a novel Pseudomonas sp. 2B. Colloids Surf B 95:23–29

    Article  CAS  Google Scholar 

  18. Rufino RD, Luna JM, Takaki GMC, Sarubbo LA (2014) Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988. Electron J Biotechnol 17:34–38

    Article  Google Scholar 

  19. Govindammal M (2014) Effect of Carbon and Nitrogen Sources on the Production of Biosurfactant by Pseudomonas fluorescens isolated from Mangrove Ecosystem. Int J Pharm Biol Arch 5:108–115

    Google Scholar 

  20. Bhardwaj G, Cameotra SS, Chopra HK (2015) Isolation and purification of a new enamide biosurfactant from Fusarium proliferatum using rice-bran. RSC Adv 5:54783–54792

    Article  CAS  Google Scholar 

  21. Silva EJ, Silva NMPR, Rufino RD, Luna JM, Silva RO, Sarubbo LA (2014) Characterization of a biosurfactant produced by Pseudomonas cepacia CCT6659 in the presence of industrial wastes and its application in the biodegradation of hydrophobic compounds in soil. Colloids Surf B 117:36–41

    Article  CAS  Google Scholar 

  22. Hazra C, Kundu D, Chaudhari A (2015) Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity. RSC Adv 5:2974–2982

    Article  CAS  Google Scholar 

  23. Williams K (2009) Biosurfactants for cosmetic applications: overcoming production challenges. MMG 445 Basic Biotechnol eJournal 5:78–83

    Google Scholar 

  24. Kuiper I, Lagendijk EL, Pickford R, Derrick JP, Lamers GEM, Thomas-Oates JE, Lugtenberg BJJ, Bloemberg GV (2004) Characterization of two Pseudomonas putida lipopeptide biosurfactants, putisolvin I and II, which inhibit biofilm formation and break down existing biofilms. Mol Microbiol 51:97–113

    Article  CAS  Google Scholar 

  25. Morikawa M, Hirata Y, Imanaka T (2000) A study on the structure-function relationship of lipopeptide biosurfactants. Biochim Biophys Acta Mol Cell Biol Lipids 1488:211–218

    Article  CAS  Google Scholar 

  26. Pecci Y, Rivardo F, Martinotti MG, Allegrone G (2010) LC/ESI-MS/MS characterisation of lipopeptide biosurfactants produced by the Bacillus licheniformis V9T14 strain. J Mass Spectrom 45:772–778

    Article  CAS  Google Scholar 

  27. Hathout Y, Ho YP, Ryzhov V, Demirev P, Fenselau C (2000) Kurstakins: a new class of lipopeptides isolated from Bacillus thuringiensis. J Nat Prod 63:1492–1496

    Article  CAS  Google Scholar 

  28. Kiran GS, Hema TA, Gandhimathi R, Selvin J, Thomas TA, Ravji TR, Natarajaseenivasan K (2009) Optimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3. Colloids Surf B 73:250–256

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are highly indebted to SLIET, Longowal and IMTECH, Chandigarh for providing the necessary facilities to carry out this research.

Author information

Authors and Affiliations

  1. Department of Chemistry, Sant Longowal Institute of Engineering and Technology (SLIET), Longowal, 148106, Sangrur, Punjab, India

    Garima Bhardwaj & Harish Kumar Chopra

  2. Institute of Microbial Technology (IMTECH), Sector-39-A, Chandigarh, 160036, India

    Swaranjit Singh Cameotra

Authors
  1. Garima Bhardwaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Swaranjit Singh Cameotra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Harish Kumar Chopra
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Harish Kumar Chopra.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bhardwaj, G., Cameotra, S.S. & Chopra, H.K. Biosurfactant from Lysinibacillus chungkukjangi from Rice Bran Oil Sludge and Potential Applications. J Surfact Deterg 19, 957–965 (2016). https://doi.org/10.1007/s11743-016-1857-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11743-016-1857-0

Keywords

Search

Navigation