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Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants

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Journal of Surfactants and Detergents

Abstract

In this work, the phase behavior variations of an ascorbyl palmitate (Asc16) system in aqueous solution were analyzed when immunologically active hydrophilic compounds (CpG and OVA) were introduced. This study was carried out through optical polarizing microscopy (OPM) and differential scanning calorimetry (DSC) at different temperatures and over a broad range of concentrations. The combination of both techniques allowed the determination of a complete phase diagram which was compared with those built for Asc16-water system and it was demonstrated that fixed concentrations of hydrophilic compounds (300 and 24 µg/g for CpG-ODN and OVA respectively) generate two lamellar liquid crystals, a cubic liquid crystal phase, and also other aggregates. However, no changes were observed in the phase diagram in terms of formation of new mesophases. The aqueous phase behavior was also studied as a function of surfactant and temperature. DSC and Fourier transform infrared spectroscopy (FT-IR) measurements show differences in the free water and mainly in the secondary hydration layer, which confirm that the studied compounds are situated in the aqueous domain. The construction and analysis of Asc16 phase diagrams with a fixed concentration of CpG-ODN/OVA allows the comprehension of Asc16 phase behavior and could be easily adapted to other concentrations. Moreover, these findings could be extrapolated to other hydrophilic substances in aqueous solution introduced in liquid crystal phases since they follow a similar behavior as those reported in the literature.

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Acknowledgments

The authors would like to thank Dra. Olga Pieroni, (Organic Chemistry Laboratory-Chemistry department from UNS.INQUISUR-CONICET Bahia Blanca, Argentina) for assistance with the IR analysis. G. Ullio Gamboa thanks the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) for a research fellowship. This work was supported by the grants SECyT-UNC. Res. 162/12 and PID CONICET No. 11220090100673 and a grant of the Universidad Nacional del Sur. LAB, and DAA are researchers of CONICET.

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    Authors and Affiliations

    1. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA-CONICET), 5000 HUA, Córdoba, Argentina

      Gabriela V. Ullio Gamboa & Daniel A. Allemandi

    2. Departamento de Farmacia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 5000 HUA, Córdoba, Argentina

      Gabriela V. Ullio Gamboa & Daniel A. Allemandi

    3. Departamento de Química, Universidad Nacional del Sur, 8000, Bahía Blanca, Argentina

      Luciano A. Benedini & Pablo C. Schulz

    4. Instituto de Química del Sur (INQUISUR-CONICET), 8000, Bahía Blanca, Argentina

      Luciano A. Benedini

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    1. Gabriela V. Ullio Gamboa
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    Correspondence to Daniel A. Allemandi.

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    Gabriela V. Ullio Gamboa and Luciano A. Benedini contributed equally to this work.

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    Ullio Gamboa, G.V., Benedini, L.A., Schulz, P.C. et al. Phase Behavior of Ascorbyl Palmitate Coagels Loaded with Oligonucleotides as a New Carrier for Vaccine Adjuvants. J Surfact Deterg 19, 747–757 (2016). https://doi.org/10.1007/s11743-016-1816-9

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    • DOI: https://doi.org/10.1007/s11743-016-1816-9

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