Ito, S., Kawata, J., Kameda, M. et al. J Surfact Deterg (2016) 19: 421. doi:10.1007/s11743-015-1771-x
Sodium laureth sulfate and amino acid type surfactants have been categorized as low-skin irritation chemicals based on results of previous skin irritation tests. However, detergent-induced skin irritation still occurs. Detergents with a low-skin irritation effect are required, since the number of cosmetics for sensitive skin has increased. Therefore, an in vitro method of testing the safety of cosmetics for sensitive skin is required. Skin irritation by anionic surfactants was investigated to determine the effect of laureth-3 carboxylate (polyoxyethylene lauryl carboxymethyl ether) amino acid salt on skin irritation. In addition, sodium laureth sulfate and amino acid type surfactants, regarded as low-irritation surfactants, were also tested for skin irritation. The skin irritation effect of laureth-3 carboxylate lysine salt (Surfactant 1), sodium laureth sulfate (Surfactant 2), and sodium N-lauroyl glutamate (Surfactant 3) were investigated using a reconstructed human cultured epidermal model, LabCyte EPI-MODEL24 6D. Cell viabilities of cultured epidermal cells exposed to Surfactant 1 (5.0 % aq.), Surfactant 2, and Surfactant 3 were 82.0, 45.0 and 19.1 %, respectively. There were significant differences in cell viability upon exposure to 5.0 % aqueous test solutions of the three test chemicals. The results of the current investigation indicate that Surfactant 1 has a low skin-irritation effect.
Laureth-3 carboxylate amino acid salt Anionic surfactant Skin irritation LabCyte EPI-MODEL 6-Day cultured model