Abstract
In the present study, the extraction and purification of saponins from Sapindus mukorossi Gaertn. were examined for effective utilization of the saponin resource. Saponins were extracted from S. mukorossi Gaertn. using water. The conditions of the water extraction process, including extraction temperature, extraction time, number of times of extraction, and solvent-material ratio were optimized. The yield of total Sapindus saponins (TSS) from the pericarp was 33.41 % and its purity in the extract was 45.71 %. The saponin solution was further concentrated to 1/6–1/7 of its original volume, and dried yeast BV818 that adapted to the concentrated Sapindus saponins solution (SW) was screened. The activation conditions, inoculum amount, fermentation temperature, and fermentation period were optimized. By using the dried yeast under optimized conditions, the purity was increased to 75.50 %. The yield of the byproduct ethanol was 5.33 % (w/v), while the content of TSS in the final product decreased from 18.29 to 15.30 % (w/v). These results could contribute to the development of industrial-scale production of Sapindus saponins.
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Acknowledgments
This article was supported by the key project of transformation and industrialization of scientific and technological achievement in Fujian Province (No. 2012N3008), the National Natural Science Foundation of People’ Republic of China (No. 21203076), Open Project of State Key Laboratory of Supramolecular Structure and Materials (sklssm 201328), Jiangsu province science and technology support program for social development (BE2012631), the Fundamental Research Funds for the Central Universities (JUSRP51309A), National Key Technology R&D Program in the 12th Five year Plan of China (No. 2012BAD36B02, 2014BAD04B03) and the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD). The manuscript has been edited by International Science Editing.
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Heng, W., Ling, Z., Na, W. et al. Extraction and Fermentation-Based Purification of Saponins from Sapindus mukorossi Gaertn.. J Surfact Deterg 18, 429–438 (2015). https://doi.org/10.1007/s11743-015-1668-8
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DOI: https://doi.org/10.1007/s11743-015-1668-8