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LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.)

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Abstract

Light-emitting diodes (LEDs) are useful for the growth of many plants, but not known for blueberry species. This study examined the effects of fluorescent lamps and 100 % red, 80 % red plus 20 % blue, 50 % red plus 50 % blue, and 100 % blue LEDs on the growth and development of highbush blueberry shoots under aseptic and non-aseptic conditions. Results revealed that monochromatic blue LEDs accumulated the highest contents of leaf chlorophylls. In contrast, monochromatic red LEDs inhibited chlorophyll accumulation, but produced the longest shoots and roots and provided high percentages of side shoot formation from ex vitro plants. Mixed LEDs, particularly 50 % red plus 50 % blue light, improved plant growth with respect to notably increased shoot and root biomass. Direct rooting of in vitro shoots under non-aseptic conditions was readily achieved using a commercial mixture of perlite and peat moss with high humidity controls. These findings obviously suggest the efficient use of LEDs to replace traditional fluorescent lamps in large-scale propagation of the highbush blueberry, and also pave the way for future studies on LEDs for standardizing micropropagation protocols to shrub crops and woody plants.

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Abbreviations

B:

100 % blue LEDs

FL:

Fluorescent lamps

LEDs:

Light-emitting diodes

R:

100 % red LEDs

R5B5:

50 % red plus 50 % blue LEDs

R8B2:

80 % red plus 20 % blue LEDs

MS:

Murashige and Skoog

MW:

Medium containing 50 % MS plus 50 % WPM

WPM:

Woody Plant Medium

ZR:

Zeatin riboside

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Acknowledgments

This work, as part of a postdoctoral research project, was funded by the Korea Ministry of Trade, Industry and Energy under the Industrial Technology Research Program (No. N0000004) through the LED Agri-Bio Fusion Technology Research Center of the Chonbuk National University, Iksan, South Korea.

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Authors and Affiliations

  1. LED Agri-bio Fusion Technology Research Center, Chonbuk National University, Iksan Campus, 79 Gobong-ro, Iksan-si, Jeollabuk-do, 570–752, Republic of Korea

    Cao Dinh Hung, Chang-Hee Hong, Seon-Ki Kim, Kyu-Han Lee, Jea-Young Park, Min-Woo Nam, Dae-Ho Choi & Hye-In Lee

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  1. Cao Dinh Hung
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  2. Chang-Hee Hong
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  3. Seon-Ki Kim
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  4. Kyu-Han Lee
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  5. Jea-Young Park
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  6. Min-Woo Nam
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  7. Dae-Ho Choi
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  8. Hye-In Lee
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Correspondence to Cao Dinh Hung or Chang-Hee Hong.

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Communicated by J. van Huylenbroeck.

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Hung, C.D., Hong, CH., Kim, SK. et al. LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.). Acta Physiol Plant 38, 152 (2016). https://doi.org/10.1007/s11738-016-2164-0

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  • DOI: https://doi.org/10.1007/s11738-016-2164-0

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