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Characterization of eight CBL genes expressions in maize early seeding development

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Abstract

Calcium (Ca2+) has been firmly established as ubiquitous second messengers functioning in plant growth development and response to environmental conditions. Calcineurin B-like (CBL) proteins, a unique group of calcium sensors, play a key role in plant response to various abiotic stresses. Here, eight ZmCBLs genes were retrieved. In terms of the gene structure, maize CBL gene had greater variability compared with rice and Arabidopsis CBLs. Phylogenetic analysis revealed that ZmCBL proteins display a close relation to OsCBLs and a little far relation to AtCBLs. Expression analysis indicated that all the eight ZmCBLs expressions were regulated by low potassium and in a tissue-dependent manner. In general, the ZmCBLs expressions in roots were more sensitive under low potassium environment, especially for ZmCBL5, 6 and 8. In leaves, only ZmCBL3, 8 and 10 expressions were upregulated. Moreover, the expression patterns of the ZmCBLs in tissues of germinated seeds and seedlings were also analyzed. The results showed that all the expressions of ZmCBLs were tissue specific except for ZmCBL6, suggesting that they may involve in seed germination and seedlings’ early growth.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31100200), the Fund of the State Key Laboratory of Crop Biology in Shandong Agricultural University (2014KF04) and the technology Key Project of the Henan Educational Committee (14A180005).

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Authors and Affiliations

  1. State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Life Science, Henan Agricultural University, 63, Road Nongye, Zhengzhou, 450002, China

    Chuntian Wang, Zhiheng Yuan, Shipeng Li, Wei Wang, Ruili Xue & Fuju Tai

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  1. Chuntian Wang
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  2. Zhiheng Yuan
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  3. Shipeng Li
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  4. Wei Wang
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Correspondence to Fuju Tai.

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Communicated by M. Hajduch.

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Wang, C., Yuan, Z., Li, S. et al. Characterization of eight CBL genes expressions in maize early seeding development. Acta Physiol Plant 36, 3307–3314 (2014). https://doi.org/10.1007/s11738-014-1698-2

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  • DOI: https://doi.org/10.1007/s11738-014-1698-2

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