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Phytotoxic effects of Heterothalamus psiadioides (Asteraceae) essential oil on adventitious rooting

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Abstract

Essential oils have many functions in plant development; among them, they take part in plant–plant interactions, such as allelopathy. The phytotoxicity of the essential oil of Heterothalamus psiadioides was evaluated on Arabidopsis thaliana adventitious rooting. This development process has demonstrated great sensitivity to phytotoxic essential oils and has been primarily studied by our research group. The essential oil of H. psiadioides showed highly negative effects on A. thaliana wild type (WT) adventitious rooting in very small amounts. As the hormone auxin has an important role on adventitious rooting, A. thaliana mutant superroot (sur1) that overproduces indole-3-acetic acid was tested to verify if it could better respond to the oil than WT. The mutant had a WT-like rooting response and the expression of its phenotype was not evident in treatments that had oil application. The essential oil also induced the generation of H2O2, a reactive oxygen species in high levels and trying to recover these effects with the aid of the antioxidant Trolox® was unsuccessful. Increasing levels of H2O2 may affect leaf pigmentation of WT microcuttings and auxin levels of both WT and sur1 microcuttings. Since the expression of auxin-responsive genes is decreased by H2O2 treatment via mitogen-activated protein kinase activation, microcuttings growth and rooting are impaired causing altered development pattern.

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Acknowledgments

We thank the Brazilian Funding agency Comissão de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the scholarship granted to Diana Carla Lazarotto, and the Laboratório de Fisiologia Vegetal (UFRGS).

Conflict of interest

All authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Botânica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    Diana Carla Lazarotto, Ângela Pawlowski, Eliane Regina da Silva & Geraldo Luiz Gonçalves Soares

  2. Departamento de Botânica, Laboratório de Ecologia Química e Quimiotaxonomia (LEQTAX), Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, Porto Alegre, RS, Brazil

    Diana Carla Lazarotto, Ângela Pawlowski, Eliane Regina da Silva & Geraldo Luiz Gonçalves Soares

  3. Centro de Ciências Agrárias e Biológicas, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas 1130, Caxias do Sul, RS, Brazil

    Joséli Schwambach

Authors
  1. Diana Carla Lazarotto
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  2. Ângela Pawlowski
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  3. Eliane Regina da Silva
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  4. Joséli Schwambach
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  5. Geraldo Luiz Gonçalves Soares
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Corresponding author

Correspondence to Diana Carla Lazarotto.

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Communicated by A. Gniazdowska-Piekarska.

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Lazarotto, D.C., Pawlowski, Â., da Silva, E.R. et al. Phytotoxic effects of Heterothalamus psiadioides (Asteraceae) essential oil on adventitious rooting. Acta Physiol Plant 36, 3163–3171 (2014). https://doi.org/10.1007/s11738-014-1682-x

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  • DOI: https://doi.org/10.1007/s11738-014-1682-x

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