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Cambium reactivation independent of bud unfolding involves de novo IAA biosynthesis in cambium regions in Populus tomentosa Carr.

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Abstract

Cambium reactivation of temperate trees in spring is important for adaptation and growth. In some trees, the resumption of cambium cell division occurs before bud flush. However, knowledge about the source of the internal stimuli that promote cambium cell division during reactivation in these trees is limited. Here, the anatomical changes of cambium during reactivation along with the unfolding of vegetative buds in Populus tomentosa were examined under natural and water-culture conditions. Meanwhile, the levels of free indole-3-acetic acid (IAA) and transcripts of enzymes involved in its production were measured during cambium reactivation. The results showed that cambium cell division occurred before bud unfolding during cambium reactivation in P. tomentosa. Interestingly, the levels of IAA and the transcripts of poplar homologs of aldehyde oxidase 2 and nitrilases 2 encoding enzymes functioning in de novo IAA biosynthesis increased before bud unfolding, while the transcript levels of IAA conjugate hydrolases almost showed no increase. These results suggest that free IAA from de novo biosynthesis in cambium regions promotes cambium reactivation before bud unfolding, providing new insights into the regulatory mechanism of cambium reactivation.

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Acknowledgments

This work was supported by the National Key Basic Research Program of China (2012CB114500) and the National Natural Science Foundation of China (31270219, 31200464). The authors thank Ms. Hua-Jun Chen and Wen-Hong Deng (Beijing Forestry University) for help in IAA measurement, Ms. Ai-Qin Song (Peking University) for statistical and image analysis, and Dr. IC Bruce (Zhejiang University) for critical reading of the manuscript.

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  1. State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, People’s Republic of China

    Wan-Feng Li, Qi Ding, Ke-Ming Cui & Xin-Qiang He

  2. Laboratory of Cell Biology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, People’s Republic of China

    Wan-Feng Li

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  1. Wan-Feng Li
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  2. Qi Ding
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Correspondence to Xin-Qiang He.

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Communicated by J. van Staden.

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Li, WF., Ding, Q., Cui, KM. et al. Cambium reactivation independent of bud unfolding involves de novo IAA biosynthesis in cambium regions in Populus tomentosa Carr.. Acta Physiol Plant 35, 1827–1836 (2013). https://doi.org/10.1007/s11738-013-1220-2

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