Abstract
In order to compare the ginsenoside composition in native Panax quinquefolium and in suspension cultured cells derived from root callus, HPLC–ESI-MSn analysis was performed. Under the present HPLC–ESI-MSn conditions, ten ginsenosides from native root were acquired in the positive and negative ion modes, namely Rg1, Re, Ro, malonyl-Rb1, Rf, Rb1, Rc, Rb2, Rb3 and Rd. Only four ginsenosides (Rg1, Re, Rf and Rb1) were identified from callus cells. Radical scavenging activity of P. quinquefolium callus cells with 250 mg l−1 methanolic extract on 1,1-diphenyl-2-picrylhydrazyl (DPPH) was 55.72 %, while only 6.31 % DPPH inhibition was obtained in native root.
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Abbreviations
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- KT:
-
Kinetin
- t R :
-
Retention times
- DPPH:
-
1,1-Diphenyl-2-picrylhydrazyl
References
Ali MB, Yu KW, Hahn EJ, Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity, ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng C.A. Mayer and Panax quinquefolium L. in bioreactor subjected to methyl jasmonate stress. Plant Sci 169:83–92
Gao WY, Jia W, Duan HQ, Xiao PG (2003) Industrialization of medicinal plant tissue culture. China J Chin Materia Medica 28:385–390
Huang T, Gao WY, Wang J, Zhao YX, Huang LQ, Liu CX (2010) Cultivation and quality assessment of tissue cultures in Panax ginseng C.A. Meyer. Minerva Biotecnol 22:39–45
Kevers C, Jacques P, Thonat P, Gasphar TH (1999) In vitro root cultures of Panax ginseng and P. quinquefolium. Plant Growth Regul 27:173–178
Kim SJ, Murthy HN, Hahn EJ, Lee HL, Paek KY (2008) Effect of processing methods on the concentrations of bioactive components of ginseng (Panax ginseng C.A. Meyer) adventitious roots. Food Sci Technol 41:959–964
Man S, Gao W, Zhang Y, Wang J, Zhao W, Huang L, Liu C (2010) Qualitative and quantitative determination of major saponins in Paris and Trillium by HPLC–ELSD and HPLC–MS/MS. J Chromatogr B 878:2943–2948
Mathur A, Mathur AK, Sangwan RS, Gangwar A, Uniyal GC (2003) Differential morphogenetic responses, ginsenoside metabolism and RAPD patterns of three Panax species. Genet Resour Crop Evol 50:245–252
Matsingou TC, Petrakis N, Kapsokefalou M, Salifoglou A (2003) Antioxidant activity of organic extracts from aqueous infusions of sage. J Agric Food Chem 51:6696–6701
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nocerino E, Amato M, Izzo AA (2000) The aphrodisiac and adaptogenic properties of ginseng. Fitoterapia 71:s1–s5
Sun BS, Pan FY, Sung CK (2011) Repetitious steaming-induced chemical transformations and global quality of black ginseng derived from Panax ginseng by HPLC–ESI-MS/MSn based chemical profiling approach. Biotechnol Bioprocess Eng 169:56–965
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Communicated by M. H. Walter.
H. Liu is the co-first author.
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Wang, J., Liu, H., Gao, WY. et al. Comparison of ginsenoside composition in native roots and cultured callus cells of Panax quinquefolium L.. Acta Physiol Plant 35, 1363–1366 (2013). https://doi.org/10.1007/s11738-012-1151-3
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DOI: https://doi.org/10.1007/s11738-012-1151-3