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Comparative study of electroacupuncture and moxibustion in influencing Tianshu (ST 25) regions mast cells in visceral hyperalgesia rats

电针与艾灸对内脏高敏感大鼠天枢穴区肥大细胞影响的对比研究

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Abstract

Objective

To evaluate and compare electroacupunctures (EA) with different parameters and moxibustion at different temperatures influencing the activation of mast cells (MC) in Tianshu (ST 25) regions of visceral hyperalgesia model rats.

Methods

Rats (except for model group) respectively accepted 1 mA or 3 mA EA or moxibustion at 43 °C or 4 °C to stimulate Tianshu (ST 25) points after randomization of the fifty visceral hyperalgesia model rats, and then were compared with that in model and normal groups. Number, degranulation numbers, degranulation rates in Tianshu (ST 25) regions MC of rats in each group were observed using toluidine blue staining. Abdominal withdrawl reflex (AWR) score was used to evaluate the rat visceral hyperalgesia reactions.

Results

Compared with the normal group and the model group, MC numbers (P<0.05, P<0.01, P<0.01, P<0.01), degranulation numbers and degranulation rates (P<0.01, P<0.01, P<0.05, P<0.01) of Tianshu (ST 25) MC in regions tissues in 43 °C and 4 °C moxibustion groups, and 1 mA and 3 mA EA groups all increased significantly. Compared with the model group, AWR scores were significantly lower in 43 °C and 4 °C moxibustion groups, and 1 mA and 3 mA EA groups under the stimulation of 20 mmHg, 40 mmHg, 0 mmHg or 80 mmHg colorectal distension (CRD) (P<0.05 in 1 mA and 3 mA EA groups under the stimulation of 20 mmHg, P<0.01 in the other groups). AWR scores in 43 °C and 4 °C oxibustion groups under the stimulation of 20 mmHg, 40 mmHg, 0 mmHg or 80 mmHg CRD were not significantly different from those in the normal group (all P>0.05); AWR scores in 1 mA EA group under the stimulation of 0 mmHg or 80 mmHg were significantly higher than that in the normal group (P<0.01); AWR score in 3 mA EA group under the stimulation of 0 mmHg was significantly higher than that in the normal group (P<0.01), and AWR scores in 3 mA EA group under the stimulation of 20 mmHg or 80 mmHg were also higher than that in the normal group (P<0.05). AWR scores were higher in 1 mA EA group under the stimulation of 40 mmHg or 80 mmHg than that in 4 °C moxibustion group (P<0.05); AWR score was higher in 3 mA EA group under the stimulation of 40 mmHg than that in 4 °C moxibustion group (P<0.05).

Conclusion

There are differences among EA of different parameters and moxibustion of different temperatures in activating on Tianshu (ST 25) regions MC of visceral hyperalgesia model rats, as well as in improving the visceral hyperalgesia reaction. The effect of 4 °C moxibustion is the most significant.

摘要

目的

观察并比较不同参数电针和不同温度艾灸对内脏高敏感模型大鼠穴区肥大细胞(mast cells, MC)活化的影响。

方法

将50 只内脏高敏感模型大鼠随机分组后分别给予1 mA、3 mA电针和43 ℃、4 ℃艾灸刺激天枢穴, 并与模型组和正常大鼠进行对照, 采用甲苯胺蓝染色法观察各组大鼠天枢穴区肥大细胞MC数量、脱颗粒数、脱颗粒率情况, 同时采用腹部撤回反射(abdominalwithdrawl reflex, AWR)评分评价各组大鼠的内脏高敏感反应。

结果

与正常组和模型组比较, 艾灸43 ℃组、艾灸4 ℃组、电针1 mA组及电针3 mA组大鼠穴区组织MC个数有显著性增加(P<0.05, P<0.01, P<0.01, P<0.01), 大鼠穴区组织MC脱颗粒数和脱颗粒率均有显著性增加 (P<0.01, P<0.01, P<0.05, P<0.01)。与模型组相比, 在20 mmHg、40 mmHg、 0 mmHg、80 mmHg结直肠扩张(colorectal distension, CRD)刺激下, 艾灸43 ℃组、艾灸4 ℃组、电针1 mA组和电针3 mA组的AWR评分均显著降低(20 mmHg刺激下电针1 mA 和电针3 mA组, P<0.05, 其余均P<0.01); 艾灸4 ℃组、艾灸43 ℃组大鼠在CRD刺激强度为20 mmHg、40 mmHg、 0 mmHg、80 mmHg时与正常组的AWR评分均无统计学差异(均P>0.05); 电针1 mA组在 0 mmHg和80 mmHg时AWR评分均显著高于正常组(均P<0.01); 电针3 mA组在 0 mmHg时AWR评分亦显著高于正常组(P<0.01), 在20 mmHg和80 mmHg时亦高于正常组(均P<0.05); 在40 mmHg和80 mmHg时, 电针1 mA组AWR评分高于艾灸4 ℃组(均P<0.05); 电针3 mA组在40 mmHg时AWR评分高于艾灸4 ℃组(P<0.05)。

结论

不同参数电针和不同温度艾灸刺激对内脏高敏感模型大鼠穴区肥大细胞活化的影响以及改善其内脏高敏感反应存在差异, 其中以4 ℃的艾灸效应最显著。

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Authors and Affiliations

  1. Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China

    Liu Chen  (陈柳), Ji-meng Zhao  (赵继梦), Yu-wei Li  (李雨薇), Hai-xia Shang  (商海霞), Lu-yi Wu  (吴璐一), Yin Shi  (施茵), Chuan-zi Dou  (窦传字) & Huan-gan Wu  (吴焕淦)

  2. Shanghai Research Institute of Acupuncture and Meridian, Shanghai, 200030, China

    Ji-meng Zhao  (赵继梦), Yin Shi  (施茵), Chuan-zi Dou  (窦传字) & Huan-gan Wu  (吴焕淦)

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  1. Liu Chen  (陈柳)
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Correspondence to Yin Shi  (施茵).

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Chen, L., Zhao, Jm., Li, Yw. et al. Comparative study of electroacupuncture and moxibustion in influencing Tianshu (ST 25) regions mast cells in visceral hyperalgesia rats. J. Acupunct. Tuina. Sci. 14, 242–249 (2016). https://doi.org/10.1007/s11726-016-0932-4

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  • DOI: https://doi.org/10.1007/s11726-016-0932-4

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