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Effects of wrist-ankle acupuncture on associated factors in uterus tissue and serum in rats with primary dysmenorrhea

腕踝针对原发性痛经大鼠子宫组织及血清相关因子的影响

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Abstract

Objective

To observe effects of wrist-ankle acupuncture (WAA) on β-endorphin (EP), nitric oxide (NO) in uterus tissue and prostaglandin F2α (PGF2α), substance P (SP) in serum of rats with primary dysmenorrhea.

Methods

A total of 45 non-pregnant Wistar rats were randomly divided into a control group, a model group and a WAA group, 15 rats in each group. Rats in the model group and the WAA group received continuous abdominal subcutaneous injection of Diethylstilbestrol to establish dysmenorrhea rat models. On the first day after modeling, rats in the WAA group began to receive acupuncture on Point Lower 1 and Point Lower 2, once a day for 10 d. The control group and the model group didn’t receive any treatment. Writhing latencies and frequencies were recorded. β-EP and NO in uterus tissue homogenates and PGF2α, SP in serum were detected.

Results

In the model group, β-EP and NO levels were the lowest among the groups, the serum PGF2α level was the highest, and serum SP level was the lowest. These measurements showed significantly difference between the model group and the control group (P<0.05). PGF2α in the WAA group was lower than that in the model group; β-EP, NO and SP levels were higher than those in the model group, with inter-group statistically significant differences (P<0.05).

Conclusion

WAA may achieve analgesic effect through decreasing PGF2α, increasing β-EP, NO and SP to relieve uterine cramps, increase blood flow and promote functional improvement.

摘要

目的

观察腕踝针对原发性痛经大鼠子宫组织匀浆中β-内啡肽(β-endorphin, EP)、一氧化氮(nitric oxide, NO)及血清中前列腺素F2α (Prostaglandin F2α, PGF2α)、P 物质(Substance P, SP)的影响。

方法

将45 只未孕Wistar 大鼠按随机数字表法分为空白对照组、模型组、腕踝针组, 每组15 只。除空白对照组外, 其余两组在大鼠腹部皮下连续注射已烯雌酚建立痛经模型, 腕踝针组造模成功后第1 天起针刺下1 区、下2 区, 每日1 次, 连续10 天。空白对照组和模型组不予任何治疗。观察大鼠的扭体潜伏期与扭体次数, 检测子宫组织匀浆中β-EP、NO 及血清中PGF2α、SP 含量。

结果

模型组大鼠子宫组织匀浆中β-EP、NO 水平最低, 血清中的PGF2α水平最高, SP 含量最低, 与空白对照组比较, 组间差异有统计学意义(P<0.05)。腕踝针组PGF2α 低于模型组, β-EP、NO、SP 水平高于模型组, 组间差异有统计学意义(P<0.05)。

结论

腕踝针可能通过抑制PGF2α, 上调β-EP、NO 及SP 缓解子宫痉挛, 增加血流量, 促进子宫组织功能改善, 达到止痛的效果。

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Authors and Affiliations

  1. Acupuncture and Tuina Department, Tangshan Hospital of Traditional Chinese Medicine, Hebei, 063000, China

    Wei-xing Liu  (刘卫星)

  2. College of Traditional Chinese Medicine, Hebei United University, Hebei, 063000, China

    Yan Zhao  (赵岩) & Yue-yue Yu  (虞跃跃)

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  1. Wei-xing Liu  (刘卫星)
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  2. Yan Zhao  (赵岩)
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  3. Yue-yue Yu  (虞跃跃)
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Correspondence to Yan Zhao  (赵岩).

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Liu, Wx., Zhao, Y. & Yu, Yy. Effects of wrist-ankle acupuncture on associated factors in uterus tissue and serum in rats with primary dysmenorrhea. J. Acupunct. Tuina. Sci. 13, 146–149 (2015). https://doi.org/10.1007/s11726-015-0839-5

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